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Series GSE113319 Query DataSets for GSE113319
Status Public on Jul 24, 2019
Title Specific combinatorial mutations in MIR396 genes enlarge the grain size and modulate the plant architecture in rice
Organism Oryza sativa
Experiment type Expression profiling by high throughput sequencing
Summary miR396 is a key growth regulator in plants, however, the molecular mechanisms underlying its functions remained to be revealed. Here, through systematically gene-editing, we found that among the MIR396 family genes, MIR396e and -f were the main regulators of rice growth. mir396ef mutations could increase the grain yield through significantly enlarging the grain size. In addition, mir396ef mutations promoted the seedling growth and modulated the plant architecture by promoting the elongations of leaves (including leaf blades and sheaths) and panicles but suppressing the elongation of internodes, especially the uppermost internode. Our research reveals that mir396ef mutations promote the growth and organ elongation by significantly increasing the level of the gibberellin (GA) precursor, mevalonic acid (MVA), which subsequently activates the GA pathway. Our results also indicate that miR396 regulates the internode elongation through a different mechanism (probably through controlling SD37 expression) from the GA pathway. These results reveal two pathways by which miR396 regulates rice growth and provide valuable gene-editing targets to increase rice productivity.
 
Overall design RNA-seq using the seedling shoots, developing uppermost internodes and young panicles (0.6–0.8 mm) of the wild type and mir396ef mutations. Two independent lines of mir396ef were used in the the transcriptome analyses of the seedling shoots. There are three biological replicates
 
Contributor(s) Miao C, Wang D
Citation(s) 31336020
Submission date Apr 18, 2018
Last update date Oct 23, 2019
Contact name Dong Wang
E-mail(s) dongwang@ncu.edu.cn
Phone (86) 15921240757
Organization name Nanchang University
Street address Hong Gu Tan New Road, No. 999
City Nanchang
State/province JiangXi
ZIP/Postal code 330031
Country China
 
Platforms (1)
GPL24468 HiSeq X Ten (Oryza sativa)
Samples (15)
GSM3103229 wild-type seedling shoot [XS134]_1
GSM3103230 wild-type seedling shoot [XS134]_2
GSM3103231 wild-type seedling shoot [XS134]_3
Relations
BioProject PRJNA450806
SRA SRP140884

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Supplementary file Size Download File type/resource
GSE113319_WTPSvsC75PS.DiffExpGene.xlsx 59.6 Kb (ftp)(http) XLSX
GSE113319_XS134vsD371.DiffExpGene.xlsx 157.1 Kb (ftp)(http) XLSX
GSE113319_XS134vsD389.DiffExpGene.xlsx 302.8 Kb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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