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| Status |
Public on Apr 08, 2009 |
| Title |
DNA interactions with the ribosomal enhancer |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
DNA-DNA interactions are important for the control of gene expression. However, DNA interactions that occur in trans are difficult to identify. We used 4Cs to identify DNA regions that interact with the yeast ribosomal DNA enhancer.
We used 4Cs to identify DNA regions that physically interact with the yeast ribosomal enhancer.
Keywords: 4C
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| Overall design |
Yeast cells were grown to exponential stage and cross linked, digested with Msp1, diluted and ligated. Fragments that interact with the ribosomal enhancer were amplified by nested PCR> Cut samples were used as a negative control. One set of samples were treated with phenanthroline prior to cross-linking.
8 samples, 4 sets of 2 biological replicates
Background controls: J0001.CEL, J0003.CEL, J0005.CEL, J0007.CEL
Cut-ligated: J0002.CEL, J0004.CEL, J0006.CEL, J0008.CEL
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| Contributor(s) |
O'Sullivan JM, Sontam DM, Grierson RJ, Jones B |
| Citation(s) |
19235779 |
| Submission date |
May 05, 2008 |
| Last update date |
Jan 09, 2016 |
| Contact name |
Justin M. O'Sullivan |
| E-mail(s) |
justin.osullivan@auckland.ac.nz
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| Organization name |
University of Auckland
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| Department |
Liggins Institute
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| Street address |
85 Park Road, Grafton
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| City |
Auckland |
| ZIP/Postal code |
1023 |
| Country |
New Zealand |
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| Platforms (1) |
| GPL7250 |
[Sc03b_MR] Affymetrix GeneChip S. cerevisiae Tiling 1.0R Array |
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| Samples (2) |
| GSM286405 |
cut-ligated exponential 4C library |
| GSM286406 |
cut-ligated phenanthroline exponential 4C library biological |
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| Relations |
| BioProject |
PRJNA106549 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE11340_RAW.tar |
189.7 Mb |
(http)(custom) |
TAR (of CEL, CHP, TXT) |
| Processed data provided as supplementary file |
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