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Series GSE114363 Query DataSets for GSE114363
Status Public on Sep 26, 2018
Title Discovery of a novel plant eIF4E-binding protein: Conserved Binding of eIF4E 1 (CBE1)
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Regulation of translation initiation is accomplished in diverse eukaryotes through proteins which bind to the mRNA cap-binding protein, eIF4E, and either prevent its association with eIF4G or form repressive mRNPs which exclude the translation machinery. Such mechanisms in plants, and even the presence of eIF4E-interacting proteins outside of eIF4G (and the plant-specific isoform eIFiso4G, which binds eIFiso4E) which operate in a gene regulatory manner are not known. Here we describe the Conserved Binding of eIF4E 1 (CBE1), a plant-specific protein with an evolutionarily conserved eIF4E binding motif. This protein binds eIF4E or eIFiso4E in vitro to form cap-binding complexes, and is found as a constituent of cap-binding complexes in vivo in an eIF4E-dependent manner. Mutants lacking CBE1 show dysregulation of cell cycle related transcripts, accumulating higher levels relative to wild-type plants of mRNAs encoding proteins involved in mitotic processes. These findings support CBE1 as a plant protein with the capability to form cap-binding complexes with the potential for gene regulatory activity.
Overall design poly(A) RNA fraction was purified and sequenced from homogenized 7-day-old seedling tissue, grown under long day conditions on 1% sucrose MS plates, with three biological replicates of Col0 (wild-type) or cbe1 mutant
Contributor(s) Patrick RM, Browning KS
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Submission date May 11, 2018
Last update date Sep 26, 2018
Contact name Ryan Patrick
Organization name Purdue University
Street address 625 Agriculture Mall Dr
City West Lafayette
State/province Indiana
ZIP/Postal code 47907
Country USA
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (6)
GSM3140469 Col0 rep1
GSM3140470 Col0 rep2
GSM3140471 Col0 rep3
BioProject PRJNA471077
SRA SRP145490

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Supplementary file Size Download File type/resource
GSE114363_gene_exp.diff.txt.gz 1.2 Mb (ftp)(http) TXT
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