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Series GSE114662 Query DataSets for GSE114662
Status Public on Dec 18, 2018
Title Genome wide analysis of chick limb bud tissue treated with FGF2 for 3hrs (RNA-Seq)
Organism Gallus gallus
Experiment type Expression profiling by high throughput sequencing
Summary During limb development, in addition to proximal to distal outgrowth, FGF regulates a number of factors necessary for SHH expression. To elucidate the molecules involved in FGF-mediated SHH expression we implanted FGF2 beads in HH23 chicken forelimb buds and excised the SHH-expressing tissue for transcriptome analysis. The hypothesis is that this transcriptome data along with data from SHH-expressing tissue after a 24hr treatment would highlight molecules necessary for SHH FGF-mediated SHH expression. Results from the overlap provide a short-list of SHH-related genes which require further testing to assess their role in mediating SHH expression.
 
Overall design Total RNA extracted from SHH-expressing chick limb bud tissue following 3hrs of FGF2 treatment compared to PBS-treated controls. Tissue from 15-20 limbs were pooled based on the treatments groups (PBS vs FGF) for each sample. 3 FGF and 3 PBS samples were submitted for RNA-seq analysis.
 
Contributor(s) Watson BA, Feenstra JM, Van Arsdale JM, Rai-Bhatti KS, Kim DH, Coggins AS, Mattison GL, Steinman ES, Pira CU, Gongol BR, Oberg KC
Citation(s) 29914077
Submission date May 18, 2018
Last update date Mar 19, 2019
Contact name Kerby Clyde Oberg
E-mail(s) koberg@llu.edu
Organization name Loma Linda University
Department Pathology and Human Anatomy
Lab Molecular Embryopathy
Street address 24785 Stewart Street
City Loma Linda
State/province CA
ZIP/Postal code 92350
Country USA
 
Platforms (1)
GPL19787 Illumina NextSeq 500 (Gallus gallus)
Samples (6)
GSM3146628 chicken forelimb bud_FGF2_3hr_rep1
GSM3146629 chicken forelimb bud_FGF2_3hr_rep2
GSM3146630 chicken forelimb bud_FGF2_3hr_rep3
This SubSeries is part of SuperSeries:
GSE114663 Genome wide analysis of chick limb bud tissue treated with FGF2 for 3 hrs
Relations
BioProject PRJNA472075
SRA SRP148467

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE114662_rpkmDFeByg.xls.txt.gz 579.8 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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