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| Status |
Public on Aug 09, 2018 |
| Title |
RNA sequencing of germinating Rhizopus delemar strain RA 99-880 |
| Organism |
Rhizopus delemar |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Rhizopus delemar is an invasive fungal pathogen, responsible for the frequently fatal disease mucormycosis. Germination, a crucial mechanism by which spores of Rhizopus delemar infect and cause disease, is a key developmental process that transforms the dormant spore state into a vegetative one. Understanding the molecular mechanisms which underpin this transformation may be key to controlling mucormycosis; however, the regulation of germination remains poorly understood. This study describes the transcriptional changes which take place over the course of germination.
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| Overall design |
mRNA sequenced from R. delemar germinated for: 0, 1, 2, 3, 4, 5, 6, 12, 16 and 24 hours. Three biological replicates were produced for each time point, mRNA from each sample was sequenced with Illumina HiSeq technology, with 100bp paired end reads.
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| Contributor(s) |
Sephton-Clark PS, Munoz JF, Ballou ER, Cuomo CA, Voelz K |
| Citation(s) |
30258038 |
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| Submission date |
May 23, 2018 |
| Last update date |
May 17, 2019 |
| Contact name |
Poppy Sephton Clark |
| E-mail(s) |
psephtonclark@gmail.com
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| Phone |
07538713486
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| Organization name |
University of Birmingham
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| Department |
IMI
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| Lab |
Ballou
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| Street address |
University of Birmingham
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| City |
Birmingham |
| State/province |
West Midlands |
| ZIP/Postal code |
B152TT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL25021 |
Illumina HiSeq 2000 (Rhizopus delemar) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA472797 |
| SRA |
SRP148808 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE114842_Rhiz_dele_RA_99-880_V4_ID2.cds.fasta.gz |
6.6 Mb |
(ftp)(http) |
FASTA |
| GSE114842_raw_counts_ID2.txt.gz |
649.8 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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