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Series GSE114899 Query DataSets for GSE114899
Status Public on Nov 25, 2020
Title Study of ribosome dynamics of rpl3[W255C] and its interactions with ribosome-associated chaperones
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by high throughput sequencing
Summary Proteostasis is a fundamental network of cellular pathways that ensures the optimal concentration and composition of correctly folded proteins within cells in normal and stress conditions. Among key components of this network are the molecular chaperones, which mediate protein folding but also act as modulators of protein synthesis. We have reported on a functional link between translation and de novo folding of proteins in the yeast Saccharomyces cerevisiae by uncovering a specific synthetic-lethal interaction between apparent unrelated mutant variants, the uL3[W255C] variant of the ribosomal protein uL3 and the null mutants of Zuo1 and Ssz1. Zuo1 and Ssz1 are components of the chaperone system named as ribosome-associated complex. Here, we performed a genome-wide analysis of ribosome dynamics by 5PSeq (Pelechano et al. 2015 PMID 26046441) in strains harbouring either wild-type uL3 or mutant uL3[W255C] in the presence or absence of Zuo1 or Ssz1. This method allows the study of ribosome dynamics, by sequencing 5’ phosphorylated mRNA co-translational degradation intermediates. Our results indicate that the rpl3[W255C] mutant is slightly impaired in translation elongation, defect that is significantly enhanced when combined with the deprivation of either Zuo1 or Ssz1.
 
Overall design We performed 2 biologically independent 5PSeq experiments.
 
Contributor(s) Rodríguez-Galán O, García-Gómez JJ, Rosado IV, Wei W, Steinmetz LM, Pelechano V, Kressler D, de la Cruz J
Citation(s) 33330942
Submission date May 24, 2018
Last update date Jan 19, 2021
Contact name Vicent Pelechano
E-mail(s) vicente.pelechano.garcia@ki.se
Organization name ScilifeLab - Karolinska Institutet
Department MTC
Street address Nobels väg 16
City Solna
ZIP/Postal code SE-17177
Country Sweden
 
Platforms (1)
GPL13821 Illumina HiSeq 2000 (Saccharomyces cerevisiae)
Samples (12)
GSM3154221 RPL3_rep1
GSM3154222 RPL3_rep2
GSM3154223 rpl3[W255C]_rep1
Relations
BioProject PRJNA473001
SRA SRP148910

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Supplementary file Size Download File type/resource
GSE114899_RAW.tar 55.0 Mb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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