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Status |
Public on Nov 25, 2020 |
Title |
Study of ribosome dynamics of rpl3[W255C] and its interactions with ribosome-associated chaperones |
Organism |
Saccharomyces cerevisiae |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Proteostasis is a fundamental network of cellular pathways that ensures the optimal concentration and composition of correctly folded proteins within cells in normal and stress conditions. Among key components of this network are the molecular chaperones, which mediate protein folding but also act as modulators of protein synthesis. We have reported on a functional link between translation and de novo folding of proteins in the yeast Saccharomyces cerevisiae by uncovering a specific synthetic-lethal interaction between apparent unrelated mutant variants, the uL3[W255C] variant of the ribosomal protein uL3 and the null mutants of Zuo1 and Ssz1. Zuo1 and Ssz1 are components of the chaperone system named as ribosome-associated complex. Here, we performed a genome-wide analysis of ribosome dynamics by 5PSeq (Pelechano et al. 2015 PMID 26046441) in strains harbouring either wild-type uL3 or mutant uL3[W255C] in the presence or absence of Zuo1 or Ssz1. This method allows the study of ribosome dynamics, by sequencing 5’ phosphorylated mRNA co-translational degradation intermediates. Our results indicate that the rpl3[W255C] mutant is slightly impaired in translation elongation, defect that is significantly enhanced when combined with the deprivation of either Zuo1 or Ssz1.
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Overall design |
We performed 2 biologically independent 5PSeq experiments.
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Contributor(s) |
Rodríguez-Galán O, García-Gómez JJ, Rosado IV, Wei W, Steinmetz LM, Pelechano V, Kressler D, de la Cruz J |
Citation(s) |
33330942 |
Submission date |
May 24, 2018 |
Last update date |
Jan 19, 2021 |
Contact name |
Vicent Pelechano |
E-mail(s) |
vicente.pelechano.garcia@ki.se
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Organization name |
ScilifeLab - Karolinska Institutet
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Department |
MTC
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Street address |
Nobels väg 16
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City |
Solna |
ZIP/Postal code |
SE-17177 |
Country |
Sweden |
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Platforms (1) |
GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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Samples (12)
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Relations |
BioProject |
PRJNA473001 |
SRA |
SRP148910 |