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| Status |
Public on Jul 17, 2019 |
| Title |
Aging-associated gene expression profiling in adipose tissue derived mesenchymal stem cells. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Background & Aims: Recently, energy metabolism in adipose tissue has been the subject of renewed interest, because significant energy expenditure can be induced in cells derived from white adipose tissue derived mesenchymal stem cells (Ad-MSCs) as beige adipocytes (BA), in addition to brown adipose tissue. Here we evaluated whether age-related changes in Ad-MSCs affect on impaired energy homeostasis and progressive obesity-related diseases. Materials and Methods: We isolated Ad-MSCs from subcutaneous murine fat tissues fed with control chow (C) or high-fat diet (60% fat; HF) for 12 or 24 weeks (younger or older groups, respectively). After two to three times passage, we performed the cellular characteristics analyses, which included differentiation property, flow cytometric and microarray analyses. Results: Ad-MSCs from older mice showed impaired differentiation capacity into BAs compared to the cells from younger mice. Global gene expression analysis by microarray revealed several genes involved with the browning of white adipose tissue were down-regulated according to aging. Additionally, HF feeding promoted aging-associated characteristic changes in Ad-MSCs. Conclusion: These results indicate the compensation potential of Ad-MSCs on energy homeostasis is affected by aging and dietary factors. A new approach targeting Ad-MSCs might represent a potential strategy for the prevention of obesity-related diseases.
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| Overall design |
Six-week-old male C57BL/6 mice were fed control chow (C) or high-fat diet (60% fat; HF) for 12 or 24 weeks. The Ad-MSC samples were collected from subcutaneous murine fat tissues, and compared global gene expression by AgilentMouse GE 8x60k Microarray.
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| Contributor(s) |
Nishikawa T, Shin-ichi H |
| Citation missing |
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| Submission date |
May 30, 2018 |
| Last update date |
Jul 19, 2019 |
| Contact name |
Taichiro Nishikawa |
| Phone |
+81-75-251-5519
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| Organization name |
Kyoto Prefectural University of Medicine
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| Street address |
465 Kajii-cho, Kamigyo-ku
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| City |
Kyoto |
| ZIP/Postal code |
602-8566 |
| Country |
Japan |
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| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA473647 |
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