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| Status |
Public on May 29, 2008 |
| Title |
Hs 294T Cells Treated with Elesclomol Alone or in Combination with NAC Compared to DMSO Treated |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We used microarrays to detail gene expression changes in Hs 294T human melanoma cells after treatment with elesclomol alone, or in combination with NAC, to aide in identifing the mechnism of action of elesclomol.
Keywords: treatment
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| Overall design |
Hs 294T human melanoma cells were grown in vitro. They were seeded at a low density and were left untreated, treated with the vehicle, treated with elesclomol, treated with NAC, or treated in combination with both elesclomol and NAC. The cells treated with NAC were given the NAC 30 minutes prior to additional treatments. They were incubated for 6 hours before harvesting.
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| |
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| Contributor(s) |
Kepros JF |
| Citation(s) |
18723479 |
| |
| Submission date |
May 23, 2008 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Jane Frances Kepros |
| E-mail(s) |
jane.kepros.2003@alum.bu.edu
|
| Organization name |
Synta Pharmaceuticals Corp.
|
| Department |
Biology
|
| Street address |
45 Hartwell Ave
|
| City |
Lexington |
| State/province |
MA |
| ZIP/Postal code |
02421 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (8)
|
| GSM290920 |
0.01% DMSO treated cells at time 6h, technical rep1. |
| GSM290921 |
0.01% DMSO treated cells at time 6h, technical rep2. |
| GSM290922 |
100nM elesclomol treated cells at time 6h, technical rep1. |
| GSM290923 |
100nM elesclomol treated cells at time 6h, technical rep2. |
| GSM290924 |
5mM NAC treated cells at time 6h, technical rep1. |
| GSM290925 |
5mM NAC treated cells at time 6h, technical rep2. |
| GSM290926 |
100nM elesclomol + 5mM NAC treated cells at time 6h, technical rep1. |
| GSM290927 |
100nM elesclomol + 5mM NAC treated cells at time 6h, technical rep2. |
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| This SubSeries is part of SuperSeries: |
| GSE11552 |
Hs 294T Cells Treated with Elesclomol in Combination with Paclitaxel or NAC |
|
| Relations |
| BioProject |
PRJNA109141 |
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