|
| Status |
Public on May 29, 2008 |
| Title |
100nM elesclomol treated cells at time 6h, technical rep1. |
| Sample type |
RNA |
| |
|
| Source name |
cryopreserved cells ordered from ATCC.org
|
| Organism |
Homo sapiens |
| Characteristics |
Hs 294T cell line
Human Melanoma
passage 11
|
| Treatment protocol |
3x10^6 cells/treatment were plated at a density of approx. 17,637cells/cm^2 and incubated for 3h prior to treatment. Pretreated with 5mM NAC for 30 min, as appropriate. Harvested after 6 h of treatment.
|
| Growth protocol |
Cells acquired from ATCC. Grown in vitro in 10% serum DMEM media.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAGEN Rneasy Mini Kit (#74106) was used for extraction according to the manufacturers instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Genechip Scanner 3000.
|
| Description |
Gene expression data from drug treated cells
|
| Data processing |
MAS5
|
| |
|
| Submission date |
May 23, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jane Frances Kepros |
| E-mail(s) |
jane.kepros.2003@alum.bu.edu
|
| Organization name |
Synta Pharmaceuticals Corp.
|
| Department |
Biology
|
| Street address |
45 Hartwell Ave
|
| City |
Lexington |
| State/province |
MA |
| ZIP/Postal code |
02421 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE11551 |
Hs 294T Cells Treated with Elesclomol Alone or in Combination with NAC Compared to DMSO Treated |
| GSE11552 |
Hs 294T Cells Treated with Elesclomol in Combination with Paclitaxel or NAC |
|
| Relations |
| Reanalyzed by |
GSE119087 |
