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Series GSE116151 Query DataSets for GSE116151
Status Public on Apr 23, 2019
Title Dynamic changes in the epigenetic landscape are mandatory for acinar cell reprogramming and pancreatic carcinogenesis [ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Pancreatic acinar cell reprogramming is described as a common hallmark in pancreatic regeneration and carcinogenesis, although the regulatory mechanisms are barely understood. To overcome limitations of cellular diversity posed by heterogeneous cell compositions and high stroma density in pathogenic tissue specimens, we have established a pure in vitro system mimicking the pancreatic carcinogenic sequence. With this model, we globally profiled for epigenetic and transcriptional alterations and demonstrate that regulatory key players and structural genes are extensively regulated by epigenetic mechanisms. A conditional knockout of the epigenetic repressor Ring1b showed that particularly the epigenetic silencing of acinar differentiation genes is a crucial step in acinar cell reprogramming and pancreatic cancer development. Moreover, a persistent epigenetic repression of these genes manifests tumor cell malignancy. Depletion or drug-dependent inhibition of Ring1b promoted tumor cell reprogramming towards a less aggressive phenotype offering new options for therapeutic intervention.
 
Overall design ChIP-Seq of H3K4me3, H3K3K27me3, and H2AK199ub in in different pancreatic tissues
 
Contributor(s) Benitz S, Straub T, Mahajan UM, Czemmel S, Unruh T, Wingerath B, Deubler S, Cheng T, Nahnsen S, Bruns P, Kong B, Raufels S, Ceyhan G, Mayerle J, Steiger K, Esposito I, Kleeff J, Michalski C, Regel I
Citation(s) 30954952
Submission date Jun 22, 2018
Last update date Apr 25, 2019
Contact name Tobias Straub
E-mail(s) tstraub@med.uni-muenchen.de
Organization name LMU Munich
Department Biomedical Center, Bioinformatics
Street address Großhadener Str. 9
City Martinsried
ZIP/Postal code 82152
Country Germany
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (20)
GSM3211448 H3K4me3 embryonic acinar cells
GSM3211449 H2AK119ub embryonic acinar cells
GSM3211450 Input embryonic acinar cells 1
This SubSeries is part of SuperSeries:
GSE116152 Dynamic changes in the epigenetic landscape are mandatory for acinar cell reprogramming and pancreatic carcinogenesis
Relations
BioProject PRJNA477454
SRA SRP151106

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE116151_RAW.tar 4.6 Gb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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