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Series GSE116707 Query DataSets for GSE116707
Status Public on Dec 04, 2018
Title Aging alters the epigenetic asymmetry of HSC division [scATAC-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Hematopoietic stem cells (HSCs) balance self-renewal and differentiation to maintain homeostasis. With aging, the frequency of polar HSCs decreases. Cell polarity in HSCs is controlled by the activity of the small RhoGTPase Cdc42. Here we demonstrate, using a comprehensive set of paired daughter cell analyses that include single cell 3D-confocal imaging, single cell transplants, single cell RNA-seq as well as single cell ATAC-seq, that the outcome of HSC divisions is strongly linked to the polarity status before mitosis, which is in turn determined by the level of the activity Cdc42 in stem cells. Aged apolar HSCs undergo preferentially self-renewing symmetric divisions, resulting in daughter stem cells with reduced regenerative capacity and lymphoid potential, while young polar HSCs undergo preferentially asymmetric divisions. Mathematical modeling in combination with experimental data implies a mechanistic role of the asymmetric sorting of Cdc42 in determining the potential of daughter cells via epigenetic mechanisms. Therefore, molecules that control HSC polarity might serve as modulators of the mode of stem cell division regulating the potential of daughter cells.
Overall design Sorted single cells were cultured with and without treatment in the presence of cytokines until first cell division (40-44hrs). The daughter cells were manually separated, washed with PBS and collected for ATAC sequencing.
Contributor(s) Mulaw M
Citation(s) 30235201
Submission date Jul 06, 2018
Last update date Feb 11, 2019
Contact name Medhanie Assmelash Mulaw
Organization name University of Ulm
Street address Albert-Einstein-Allee 11
City Ulm
ZIP/Postal code 89081
Country Germany
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (48)
GSM3258876 Aged_Daughter 3a1A
GSM3258877 Aged_Daughter 3a1B
GSM3258878 Aged_Daughter 3a2A
This SubSeries is part of SuperSeries:
GSE116712 Aging alters the epigenetic asymmetry of HSC division
BioProject PRJNA479966
SRA SRP152506

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Supplementary file Size Download File type/resource
GSE116707_RAW.tar 76.0 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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