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| Status |
Public on Jun 16, 2021 |
| Title |
Freeze-dried mouse sperm nucleus has tolerance against extreme environments |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To demonstrate the tolerance of mammalian sperm nucleus against extreme environments, mouse spermatozoa were freeze-dried and treated with 95 °C for 1 h or irradiated at over 5 Gy. Although all sperm were ostensibly dead after rehydration, healthy offspring were obtained from recovered sperm nuclei. The normality of those offspring were examined by microarray, and no difference were detected compare to fresh control offspring.
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| Overall design |
Neonate brain derived from fresh spermatozoa, freeze-dried (FD) spermatozoa, 5 Gy X-ray irradiated FD spermatozoa and FD spermatozoa treated at 95 °C for 1 h were used.
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| |
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| Contributor(s) |
Wakayama S, Araki R, Wakayama T |
| Citation(s) |
34117068 |
| |
| Submission date |
Jul 26, 2018 |
| Last update date |
Jun 16, 2021 |
| Contact name |
Ryoko Araki |
| E-mail(s) |
araki.ryoko@qst.go.jp
|
| Organization name |
National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology
|
| Street address |
Anagawa4-9-1, Inage-ku
|
| City |
Chiba |
| ZIP/Postal code |
263-8555 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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| Samples (16)
|
| GSM3308035 |
neonatal brain_fresh spermatozoa_rep4 |
| GSM3308036 |
neonatal brain_FD spermatozoa_control_rep1 |
| GSM3308037 |
neonatal brain_FD spermatozoa_control_rep2 |
| GSM3308038 |
neonatal brain_FD spermatozoa_control_rep3 |
| GSM3308039 |
neonatal brain_FD spermatozoa_control_rep4 |
| GSM3308040 |
neonatal brain_FD spermatozoa_5Gy_rep1 |
| GSM3308041 |
neonatal brain_FD spermatozoa_5Gy_rep2 |
| GSM3308042 |
neonatal brain_FD spermatozoa_5Gy_rep3 |
| GSM3308043 |
neonatal brain_FD spermatozoa_5Gy_rep4 |
| GSM3308044 |
neonatal brain_FD spermatozoa_95°C1h_rep1 |
| GSM3308045 |
neonatal brain_FD spermatozoa_95°C1h_rep2 |
| GSM3308046 |
neonatal brain_FD spermatozoa_95°C1h_rep3 |
| GSM3308047 |
neonatal brain_FD spermatozoa_95°C1h_rep4 |
|
| Relations |
| BioProject |
PRJNA483053 |
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