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| Status |
Public on Jun 10, 2009 |
| Title |
Muscle gene expression of lambs with maternal callipyge allele |
| Platform organism |
Bos taurus |
| Sample organism |
Ovis aries |
| Experiment type |
Expression profiling by array
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| Summary |
Lambs that inherit a callipyge allele from their dam have an up-regulation of maternally imprinted transcripts near the callipyge mutation but do not exhibit muscle hypertrophy. It is not clear what effects these maternally expressed transcripts have in the muscle or how the inheritance of a maternal callipyge allele prevents the expression of the callipyge phenotype which is seen paternal heterozygotes only.
This experiment utilized bovine gene expression arrays to profile the gene expression of the semimembranosus muscle of 30 day old half-sibling lambs of all four possible genotypes with respect to the callipyge allele (+/+, C/+, +/C, and C/C).
Keywords: allele effect
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| Overall design |
10 lambs were used for this study. N=3 biological reps for C/C genotype, N=3 biological reps for Cmat/+ genotype, N=2 biological reps for +/Cpat genotype, and N=2 biological reps for +/+ genotype. Pairwise contrasts were performed to analyze the presence of a maternally inherited callipyge allele and the effect on a homozygous animal (+/C vs CC; +/+ vs C/+; and C/+ vs C/C).
These data make up two experiments.
[1] The maternal allele (4 genotype) experiment compares all four possible genotypes of a particular allele at one age and one tissue (30 days semimembranosus).
[2] The paternal allele (2 genotype) experiment compares only the NC and the NN genotypes in one tissue (SM) across 4 ages (10, 20, 30, and 80 days).
Images were interpreted with Microarray Suite version 5.0 (MAS5.0) in GCOS with no scaling or normalization (i.e., Samples GSM298143-GSM298164). Images were also interpreted by RMA using Bioconductor package of R (i.e., Samples GSM298165-GSM298186).
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| Contributor(s) |
Fleming-Waddell JN, Olbricht GR, Taxis TM, Vuocolo T, Byrne K, Craig BA, Tellam RL, Neary MK, Cockett NE, Bidwell CA |
| Citation(s) |
19816583 |
| Submission date |
Jun 13, 2008 |
| Last update date |
Dec 17, 2012 |
| Contact name |
Christopher A Bidwell |
| E-mail(s) |
cbidwell@purdue.edu
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| Phone |
765-494-8016
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| Organization name |
Purdue University
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| Department |
Animal Sciences
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| Street address |
125 South Russell Street
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| City |
West Lafayette |
| State/province |
IN |
| ZIP/Postal code |
47907-2042 |
| Country |
USA |
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| Platforms (1) |
| GPL2112 |
[Bovine] Affymetrix Bovine Genome Array |
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| Samples (44)
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| GSM298143 |
10d SM muscle of +/Cpat, biological rep 1 |
| GSM298144 |
10d SM muscle of +/Cpat, biological rep 2 |
| GSM298145 |
10d SM muscle of +/+, biological rep 1 |
| GSM298146 |
10d SM muscle of +/+, biological rep 2 |
| GSM298147 |
20d SM muscle of +/Cpat, biological rep 1 |
| GSM298148 |
20d SM muscle of +/Cpat, biological rep 2 |
| GSM298149 |
20d SM muscle of +/+, biological rep 1 |
| GSM298150 |
20d SM muscle of +/+, biological rep 2 |
| GSM298151 |
30d SM muscle of +/Cpat, biological rep 1 |
| GSM298152 |
30d SM muscle of +/Cpat, biological rep 2 |
| GSM298153 |
30d SM muscle of +/+, biological rep 1 |
| GSM298154 |
30d SM muscle of +/+, biological rep 2 |
| GSM298155 |
30d SM muscle of Cmat/+, biological rep 1 |
| GSM298156 |
30d SM muscle of Cmat/+, biological rep 2 |
| GSM298157 |
30d SM muscle of Cmat/+, biological rep 3 |
| GSM298158 |
30d SM muscle of C/C, biological rep 1 |
| GSM298159 |
30d SM muscle of C/C, biological rep 2 |
| GSM298160 |
30d SM muscle of C/C, biological rep 3 |
| GSM298161 |
80d SM muscle of +/Cpat, biological rep 1 |
| GSM298162 |
80d SM muscle of +/Cpat, biological rep 2 |
| GSM298163 |
80d SM muscle of +/+, biological rep 1 |
| GSM298164 |
80d SM muscle of +/+, biological rep 2 |
| GSM298165 |
10d SM muscle of +/Cpat, biological rep 1 [RMA] |
| GSM298166 |
10d SM muscle of +/Cpat, biological rep 2 [RMA] |
| GSM298167 |
10d SM muscle of +/+, biological rep 1 [RMA] |
| GSM298168 |
10d SM muscle of +/+, biological rep 2 [RMA] |
| GSM298169 |
20d SM muscle of +/Cpat, biological rep 1 [RMA] |
| GSM298170 |
20d SM muscle of +/Cpat, biological rep 2 [RMA] |
| GSM298171 |
20d SM muscle of +/+, biological rep 1 [RMA] |
| GSM298172 |
20d SM muscle of +/+, biological rep 2 [RMA] |
| GSM298173 |
30d SM muscle of +/Cpat, biological rep 1 [RMA] |
| GSM298174 |
30d SM muscle of +/Cpat, biological rep 2 [RMA] |
| GSM298175 |
30d SM muscle of +/+, biological rep 1 [RMA] |
| GSM298176 |
30d SM muscle of +/+, biological rep 2 [RMA] |
| GSM298177 |
30d SM muscle of Cmat/+, biological rep 1 [RMA] |
| GSM298178 |
30d SM muscle of Cmat/+, biological rep 2 [RMA] |
| GSM298179 |
30d SM muscle of Cmat/+, biological rep 3 [RMA] |
| GSM298180 |
30d SM muscle of C/C, biological rep 1 [RMA] |
| GSM298181 |
30d SM muscle of C/C, biological rep 2 [RMA] |
| GSM298182 |
30d SM muscle of C/C, biological rep 3 [RMA] |
| GSM298183 |
80d SM muscle of +/Cpat, biological rep 1 [RMA] |
| GSM298184 |
80d SM muscle of +/Cpat, biological rep 2 [RMA] |
| GSM298185 |
80d SM muscle of +/+, biological rep 1 [RMA] |
| GSM298186 |
80d SM muscle of +/+, biological rep 2 [RMA] |
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| Relations |
| BioProject |
PRJNA105971 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE11780_RAW.tar |
81.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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