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| Status |
Public on Aug 09, 2018 |
| Title |
Placenta and uterus transcriptomes from transgenic mice expressing human corticotropin-releasing hormone in placenta |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Parturition is delayed by approximately 12 hours in transgenic mice expressing human corticotropin-releasing hormone (CRH) in placenta. The goal of the study was to identify the pathways in reproductive tissues (uterus and placenta) altered by placental expression of human CRH.
Methods: Human BAC RP11-366K18 (CHORI) containing human CRH and cis-regulatory region was inserted into the mouse genome by microinjection and random integration to create the BAC1 line. The CRISPR/Cas9 system was used to delete a CRH regulatory element from the BAC1 line to create the CR1 line, eliminating expression of CRH in placenta. Total expression of uterus and placenta by RNA-seq at embryonic day 18.5 were compared between BAC1, CR1, and nontransgenic mice.
Results: Genes known to be associated with luteolysis and initiation of parturition (Cav1, Gja1, Oxtr, Ptgs1, Ptgs2) were not differentially expressed in uterus of this model.
Conclusions: CRH-mediated delay of parturition is likely independent of luteolysis.
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| Overall design |
mRNA-seq was performed on uterus and placenta harvested at embryonic day 18.5 from nontransgenic mice, Tg(BAC1) mice, and Tg(CR1) mice.
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| Contributor(s) |
Dunn-Fletcher CE, Muglia LJ |
| Citation(s) |
30231016 |
| Submission date |
Aug 08, 2018 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Caitlin E Dunn |
| E-mail(s) |
caitlin.dunn@cchmc.org
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| Organization name |
Cincinnati Children's Hospital Medical Center
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| Department |
Division of Human Genetics
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| Lab |
Muglia Lab
|
| Street address |
3333 Burnet Avenue
|
| City |
Cincinnati |
| State/province |
Ohio |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA485077 |
| SRA |
SRP156731 |
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