|
| Status |
Public on Aug 09, 2018 |
| Title |
4568U |
| Sample type |
SRA |
| |
|
| Source name |
uterus
|
| Organism |
Mus musculus |
| Characteristics |
strain: Mixed C57BL/6 and FVB
genotype: Tg(CR1)/Tg(CR1)
|
| Treatment protocol |
Male mice were housed with females overnight to determine time of conception. Pregnant female mice were sacrificed on the designated day of gestation and tissues were harvested and flash frozen in liquid nitrogen.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was extracted with the TRIzol reagent (ThermoFisher Scientific). All samples had RQN >7 measured on an Advanced Analytical Fragment Analyzer.
PolyA stranded RNA-seq was performed on an Illumina HiSeq machine using a paired-end approach with 75 bp reads, generating approximately 30 million paired reads per sample.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Description |
UterusTPM_by_gene
4568U
|
| Data processing |
Reads were trimmed using Trimmomatic and aligned using the HiSat2 aligner.
Transcript counting and quantification was done using kallisto.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include TPM values for each sample
|
| |
|
| Submission date |
Aug 08, 2018 |
| Last update date |
Aug 09, 2018 |
| Contact name |
Caitlin E Dunn |
| E-mail(s) |
caitlin.dunn@cchmc.org
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Division of Human Genetics
|
| Lab |
Muglia Lab
|
| Street address |
3333 Burnet Avenue
|
| City |
Cincinnati |
| State/province |
Ohio |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL21493 |
| Series (1) |
| GSE118283 |
Placenta and uterus transcriptomes from transgenic mice expressing human corticotropin-releasing hormone in placenta |
|
| Relations |
| BioSample |
SAMN09783537 |
| SRA |
SRX4520626 |
