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| Status |
Public on Jun 23, 2008 |
| Title |
Global transcriptional profiling of Saccharomyces cerevisiae treated with allicin |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
We employed CapitalBio Corporation to investigate the global transcriptional profiling of Saccharomyces cerevisiae treated with allicin.
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| Overall design |
S. cerevisiae strain L1190 was incubated in a rotary shaker. Subsequently, allicin was added to the culture and DMSO to the control. Then, the yeast cells were further incubated for a certain time and total RNA was extracted by a hot acidic phenol method. The experimental sample and control sample were incorporated and dissolved in hybridization solution after labelled. Arrays hybridization was preformed in a CapitalBio BioMixerTM II Hybridization Station overnight and washed with two consecutive solutions. Arrays were scanned with a confocal LuxScanTM scanner and the images obtained were then analyzed using LuxScanTM 3.0 software. A space- and intensity-dependent normalization based on a LOWESS program was employed.
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| Citation(s) |
20617313 |
| |
| Submission date |
Jun 22, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Xuming Deng |
| E-mail(s) |
xumingdeng@yahoo.com.cn
|
| Organization name |
College of Animal Science and Veterinary Medicine, Jilin University
|
| Street address |
Xi'an Street 5333#
|
| City |
Changchun |
| ZIP/Postal code |
130062 |
| Country |
China |
| |
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| Platforms (1) |
| GPL6010 |
CapitalBio Saccharomyces cerevisiae 23K oligo array |
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| Samples (3) |
| GSM299444 |
Saccharomyces cerevisiae oligo microarray_rep1_allicin |
| GSM299445 |
Saccharomyces cerevisiae oligo microarray_rep2_allicin |
| GSM299446 |
Saccharomyces cerevisiae oligo microarray_rep3_allicin |
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| Relations |
| BioProject |
PRJNA105657 |
