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Series GSE119130 Query DataSets for GSE119130
Status Public on Jan 31, 2019
Title Rbpj expression in regulatory T cells is critical for restraining TH2 responses [lymph nodes RbpjWT and diseased RbpjKO expression]
Organism Mus musculus
Experiment type Expression profiling by array
Summary The transcriptional regulator Rbpj is involved in T-helper (TH) subset polarization, but its function in Treg cells remains unclear. Here we show that Treg-specific Rbpj deletion leads to splenomegaly and lymphadenopathy despite increased numbers of Treg cells with a polyclonal TCR repertoire. A specific defect of Rbpj-deficient Treg cells in controlling TH2 polarization and B cell responses was observed, leading to the spontaneous formation of germinal centers and a TH2-associated immunoglobulin class switch as well as T-cell polarization into TH2 effector cells. The observed phenotype was environment-dependent and could be induced by infection with parasitic nematodes. Rbpj-deficient Treg cells adopted open chromatin landscapes and gene expression profiles reminiscent of tissue-derived TH2-polarized Treg cells, with a prevailing footprint of transcription factor Gata-3. The over-expression of Ilt3 rendered Rbpj-deficient Treg cells incompatible to specifically restrain TH2 responses, finally leading to severe and fatal lymphoproliferative disease.
 
Overall design We isolated lymph nodes from affected Treg Rbpj-deficient animals and wildtype counterparts. Total RNA was isolated and subjected to gene expression analysis using Illumina Bead Chip technology
 
Contributor(s) Delacher M, Feuerer M
Citation(s) 30962454
Submission date Aug 28, 2018
Last update date Apr 17, 2019
Contact name Michael Delacher
E-mail(s) delacher@uni-mainz.de
Phone +49 6131 17 6574
Organization name University Medical Center of the Johannes Gutenberg-University Mainz
Department Institute for Immunology
Lab Immunology
Street address Langenbeckstrasse 1
City Mainz
State/province Rhineland-Palatinate
ZIP/Postal code 55131
Country Germany
 
Platforms (1)
GPL17543 Illumina MouseWG-6 v2.0 R2 expression beadchip
Samples (6)
GSM3359091 LN_WT1_985_3998574034_A
GSM3359092 LN_WT2_980_3998574034_B
GSM3359093 LN_WT3_976_3998574034_C
This SubSeries is part of SuperSeries:
GSE119169 Rbpj expression in regulatory T cells is critical for restraining TH2 responses
Relations
BioProject PRJNA488238

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE119130_RAW.tar 9.5 Mb (http)(custom) TAR (of IDAT)
GSE119130_non-normallized.txt.gz 20.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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