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| Status |
Public on Mar 09, 2010 |
| Title |
Brachyury ChIP-chip on Ciona intestinalis embryo (early-mid gastrula) |
| Organism |
Ciona intestinalis |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
A genomic overview of in vivo binding of a transcription factor Brachyury in the ascidian gastrula embryo.
Brachyury is known to be essential and sufficient for differentiation of the notochord.
Keywords: ChIP-chip
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| Overall design |
Fertilized eggs were electroporated with a construct encoding GFP-tagged Brachyury. After fixation, Chromatin was sonicated to obtain DNA fragments with an average of size of 1kb. The DNA fragments were enriched by immunoprecipitation with an polyclonal antibody specific for GFP (Invitrogen). The immunoprecipitated and WCE DNA were amplified by LM-PCR for the Chip analysis.
Two independent experiments were performed to reduce experimental errors.
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| Contributor(s) |
Satou Y, Kubo A |
| Citation(s) |
20392745 |
| Submission date |
Jun 28, 2008 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Atsushi Kubo |
| Organization name |
Kyoto University
|
| Department |
Department of zoology, Graduate School of Science
|
| Street address |
Sakyo-ku
|
| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
606-8502 |
| Country |
Japan |
| |
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| Platforms (2) |
| GPL6555 |
Kyoto University Custom Agilent Ciona intestinails 242K Tiling Array No.1 (of 2) |
| GPL6556 |
Kyoto University Custom Agilent Ciona intestinails 242K Tiling Array No.2 (of 2) |
|
| Samples (4)
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| GSM300069 |
Brachyury ChIP-chip on Ciona intestinalis embryo (early-mid gastrula) Sample 1, Array 1 |
| GSM300071 |
Brachyury ChIP-chip on Ciona intestinalis embryo (early-mid gastrula) Sample 1, Array 2 |
| GSM300471 |
Brachyury ChIP-chip on Ciona intestinalis embryo (early-mid gastrula) Sample 2, Array 1 |
| GSM300475 |
Brachyury ChIP-chip on Ciona intestinalis embryo (early-mid gastrula) Sample 2, Array 2 |
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| This SubSeries is part of SuperSeries: |
| GSE17976 |
ChIP-chip analysis of the Ciona intestinalis embryo |
|
| Relations |
| BioProject |
PRJNA123427 |
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