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Status |
Public on Sep 03, 2020 |
Title |
Intestine-to-germline transmission of epigenetic information transgenerationally ensures systemic stress resistance in C. elegans |
Organism |
Caenorhabditis elegans |
Experiment type |
Expression profiling by array
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Summary |
Epigenetic states are metastable and changed throughout life, and their changes play a major role in the regulation of organismal homeostasis, including stress resistance. However, the mechanisms coordinating epigenetic states and systemic stress resistance across tissues remain largely unknown. Here, we identify the intestine-to-germline communication of epigenetic states, which enhances stress resistance transgenerationally in C. elegans. We firstly show that the alteration in epigenetic states by deficiency of the histone H3K4me3 modifier ASH-2 in the intestine or germline increases organismal oxidative stress resistance, which is abrogated by knockdown of the H3K4 demethylase RBR-2 in the same tissue. Remarkably, the increase in stress resistance induced by ASH-2 deficiency in the intestine is abrogated by RBR-2 knockdown in the germline, suggesting the existence of inter-tissue transmission of epigenetic information from intestine to germline. Simultaneously, this intestine-to-germline communication in the parental generation transgenerationally provides the increase in stress resistance of the descendants for over two generations. Further analyses reveal that the inter-tissue communication requires the insulin/IGF-1 signaling effector DAF-16/FOXO in the intestine and is mediated, at least in part, by transcriptional regulation of F08F1.3, one of the intestinal ASH-2 target genes. These results unveil the novel intestine-to-germline communication of epigenetic information that provides the transgenerational regulation of organismal stress resistance. To further investigate the molecular mechanisms underlying intestine-to-germline communication, we sought the gene expression changes in the intestine ash-2 knockdown worms.
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Overall design |
Total RNA was isolated using TRIzol reagent (Invitrogen) in N2, VP303, NR350, TU3401 worms under Control RNAi and ash-2 RNAi conditions. Other procedures were performed according to Ambion WT cDNA Expression Kit protocols. RNA degradation and cRNA elongation were verified with an Agilent 2100 Bioanalyzer. The fragmented cRNA was hybridized using GeneChip C. elegans Gene 1.0 ST Array (Affymetrix) at 45℃ for 16 h. Hybridized arrays scanned using an Affymetrix GeneChip Scanner. Scanned chip images were analyzed with Affymetrix GeneChip Command Console version 2.0 (AGCC) and processed using default settings. The Affymetrix output (CEL files) were imported into the GeneSpring GX 11.0.2 (Agilent Technologies) microarray analysis software for the presentation of the expression profiles.
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Contributor(s) |
Nono M, Kishimoto S, Nishida E, Uno M |
Citation(s) |
32160530 |
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Submission date |
Sep 13, 2018 |
Last update date |
Dec 06, 2020 |
Contact name |
Eisuke Nishida |
E-mail(s) |
nishida@lif.kyoto-u.ac.jp
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Phone |
+81-75-753-4230
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Organization name |
Graduate School of Biostudies, Kyoto University
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Department |
Department of Cell and Developmental Biology
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Street address |
Kitashirakawa, Sakyo-ku
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City |
Kyoto |
ZIP/Postal code |
606-8502 |
Country |
Japan |
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Platforms (1) |
GPL19230 |
[EleGene-1_0-st] Affymetrix C. elegans Gene 1.0 ST Array [transcript (gene) version] |
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Samples (8)
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Relations |
BioProject |
PRJNA490709 |
Supplementary file |
Size |
Download |
File type/resource |
GSE119917_RAW.tar |
44.1 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
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