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| Status |
Public on Jul 01, 2019 |
| Title |
Multiple genes recruited from hormone pathways partition maize diterpenoid defences. |
| Organism |
Zea mays |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Duplication and divergence of primary pathway genes underlie the evolution of plant specialized metabolism; however, mechanisms partitioning parallel hormone and defence pathways are often speculative. For example, the primary pathway intermediate ent-kaurene is essential for gibberellin biosynthesis and is also a proposed precursor for maize antibiotics. By integrating transcriptional coregulation patterns, genome-wide association studies, combinatorial enzyme assays, proteomics and targeted mutant analyses, we show that maize kauralexin biosynthesis proceeds via the positional isomer ent-isokaurene formed by a diterpene synthase pair recruited from gibberellin metabolism. The oxygenation and subsequent desaturation of ent-isokaurene by three promiscuous cytochrome P450s and a new steroid 5α reductase indirectly yields predominant ent-kaurene-associated antibiotics required for Fusarium stalk rot resistance. The divergence and differential expression of pathway branches derived from multiple duplicated hormone-metabolic genes minimizes dysregulation of primary metabolism via the circuitous biosynthesis of ent-kaurene-related antibiotics without the production of growth hormone precursors during defence.
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| Overall design |
A total of 8 samples were analyzed, comprised of two treatments, SLB 24 h and a 0.1%Tween 20 in H20 control 24 h, all in replicates of four
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| Contributor(s) |
Schmitz RJ, Schmelz E |
| Citation(s) |
31527844 |
| Submission date |
Sep 18, 2018 |
| Last update date |
Oct 01, 2019 |
| Contact name |
Robert J Schmitz |
| E-mail(s) |
schmitz@uga.edu
|
| Organization name |
University of Georgia
|
| Department |
Genetics
|
| Street address |
B416 Davison Life Sciences
|
| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30602 |
| Country |
USA |
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| Platforms (1) |
| GPL20156 |
Illumina NextSeq 500 (Zea mays) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA491716 |
| SRA |
SRP162042 |
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