|
| Status |
Public on Jul 01, 2019 |
| Title |
SLB 24hr leaf treatment rep4 |
| Sample type |
SRA |
| |
|
| Source name |
Emergent but non-fully-expanded 4th leaf
|
| Organism |
Zea mays |
| Characteristics |
cultivar: Var. Golden Queen
developmental stage: V-3
tissue: Leaf
age: 14 d
|
| Treatment protocol |
N/A
|
| Growth protocol |
Zea mays Var. Golden Queen seeds were germinated in MetroMix 200 (Sun Gro Horticulture Distribution, Inc.) supplemented with 14-14-14 Osmocote (Scotts Miracle-Gro) and grown in a greenhouse with a 12-h photoperiod, minimum of 300 μmol m−2 s−1 of photosynthetically active radiation supplied by supplemental lighting, 70% relative humidity, and temperature cycle of 24 °C/28 °C (night/day).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA-seq libraries were constructed using Illumina TruSeq Stranded RNA LT Kit following the manufacturer’s instructions with limited modifications. The starting quantity of total RNA was adjusted to 1.3 µg, and all volumes were reduced to a third of the described quantity.
Stranded mRNA-seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
SLB spore suspension (1X106)
|
| Data processing |
Raw fastq reads were trimmed for adapters, preprocessed to remove low quality reads using Trimmomatic v0.32
Genome_build: Z. mays AGPv4.34
Supplementary_files_format_and_content: tab delimited text file, see "RNA_column_header_descriptions.txt" for column headers
|
| |
|
| Submission date |
Sep 18, 2018 |
| Last update date |
Jul 01, 2019 |
| Contact name |
Robert J Schmitz |
| E-mail(s) |
schmitz@uga.edu
|
| Organization name |
University of Georgia
|
| Department |
Genetics
|
| Street address |
B416 Davison Life Sciences
|
| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30602 |
| Country |
USA |
| |
|
| Platform ID |
GPL20156 |
| Series (1) |
| GSE120135 |
Multiple genes recruited from hormone pathways partition maize diterpenoid defences. |
|
| Relations |
| BioSample |
SAMN10086253 |
| SRA |
SRX4712299 |
