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| Status |
Public on Dec 10, 2018 |
| Title |
Nanostring gene expression analysis of splenocytes from 17-β estradiol (E2) and synthetic 17α-ethinyl estradiol (EE) exposed feamle NZB/WF1 lupus mice |
| Platform organism |
Mus musculus domesticus |
| Sample organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We used the NanoString mouse nCounter immunology panel platform to compare mRNA gene expression profiles of spelnocytes from placebo-, E2, and EE exposed NZB/WF1 mice. With the profling of 548 immunology-related genes, we found that E2 and EE exposure alter immune-related genes at a similar pattern.
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| Overall design |
Female NZB/WF1 lupus mice received E2, EE, or empty (placebo control) implant treatment at 6-7 weeks of age. After 9 wks of treatment, the mice were terminated and splenocytes were isolated and collected. Total RNAs were extracted from splenocytes with miRNeasy Mini Kit and sent to NanoString Technologies for gene profiling analysis with nCounter GX mouse immunology kit.
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| Contributor(s) |
Dai R, Ahmed SA |
| Citation(s) |
30418530 |
| Submission date |
Oct 24, 2018 |
| Last update date |
Mar 11, 2019 |
| Contact name |
Rujuan Dai |
| E-mail(s) |
rdai05@vt.edu
|
| Phone |
5402313966
|
| Organization name |
Virginia Tech
|
| Department |
biomedical science
|
| Street address |
IDRF 127c,, 245 duck pond drive
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| City |
Blacksburg |
| State/province |
VA |
| ZIP/Postal code |
24060 |
| Country |
USA |
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| Platforms (1) |
| GPL23907 |
UMN MCC nCounter Mouse 561 Immunology genes |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE121745 |
Nanostring expression analysis of splenocytes from 17-β estradiol (E2) and synthetic 17α-ethinyl estradiol (EE) exposed feamle NZB/WF1 lupus mice |
|
| Relations |
| BioProject |
PRJNA498303 |
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