|
| Status |
Public on Dec 10, 2018 |
| Title |
Sple-EE14 |
| Sample type |
RNA |
| |
|
| Source name |
splenocytes
|
| Organism |
Mus musculus |
| Characteristics |
strain: NZB/WF1
gender: female
treatment: 17alpha-ethinyl estradiol (EE)
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted from splenocytes with miRNeasy Mini Kit. On-column DNase digestion with RNase-free DNase (Qiagen) was performed to remove residual amounts of DNA contamination in the isolated RNA
|
| Label |
digital molecular barcode
|
| Label protocol |
According to the manufactuer's protocol. http://academicdepartments.musc.edu/cohr/l-cohr/nanostring/geneassaymanual.pdf
|
| |
|
| Hybridization protocol |
According to the manufactuer's protocol. http://academicdepartments.musc.edu/cohr/l-cohr/nanostring/geneassaymanual.pdf
|
| Scan protocol |
Cartrige were placed into the nCounter Digital Analyzer for direct digital counting
|
| Description |
Among gene expression codeset profiling 561 genes, 547 immunology-related mouse genes+14 internal reference controls were added
|
| Data processing |
The raw data in .RCC file were uploaded to nSolver Nanostring software 3.0 and processed with default quality control settings, and then for data normalization and expression analysis.
|
| |
|
| Submission date |
Oct 24, 2018 |
| Last update date |
Dec 10, 2018 |
| Contact name |
Rujuan Dai |
| E-mail(s) |
rdai05@vt.edu
|
| Phone |
5402313966
|
| Organization name |
Virginia Tech
|
| Department |
biomedical science
|
| Street address |
IDRF 127c,, 245 duck pond drive
|
| City |
Blacksburg |
| State/province |
VA |
| ZIP/Postal code |
24060 |
| Country |
USA |
| |
|
| Platform ID |
GPL23907 |
| Series (2) |
| GSE121743 |
Nanostring gene expression analysis of splenocytes from 17-β estradiol (E2) and synthetic 17α-ethinyl estradiol (EE) exposed feamle NZB/WF1 lupus mice |
| GSE121745 |
Nanostring expression analysis of splenocytes from 17-β estradiol (E2) and synthetic 17α-ethinyl estradiol (EE) exposed feamle NZB/WF1 lupus mice |
|
