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| Status |
Public on Sep 21, 2020 |
| Title |
mRNA adenosine methylase (MTA) deposits m6A on pri-miRNAs to modulate miRNA biogenesis in Arabidopsis thaliana [smallRNAs] |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
In order to assess the possible effects of m6A on miRNA biogenesis we performed small RNA (sRNA) sequencing to compare their levels in WT Col-0 plants to those in mutant lines with severely reduced m6A levels. The reduced mta line contains MTA cDNA under the ABI3 promoter in MTA null mutant background (ABI3:MTA) and ABI3 being an embryo specific promoter rescues the embryo lethal phenotype of MTA knockout while keeping the levels of MTA in mature plants severely low34. sRNAs isolated from 4 week old WT and mta plants were used to make libraries that were sequenced using HiSeq® (Illumina) and the results were analyzed using NOISeq package in R-Studio.
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| Overall design |
wt and mta mutant small RNA sequencing
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| |
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| Contributor(s) |
Bielewicz D, Jarmolowski A, Szweykowska-Kulinska Z |
| Citation(s) |
32817553 |
| Submission date |
Nov 14, 2018 |
| Last update date |
Sep 21, 2020 |
| Contact name |
Zofia Szweykowska-Kulinska |
| E-mail(s) |
zofszwey@amu.edu.pl
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| Organization name |
Adam Mickiewicz University in Poznan
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| Department |
Departemnt of Gene Expression
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| Street address |
Umultowska 89
|
| City |
Poznan |
| ZIP/Postal code |
61-614 |
| Country |
Poland |
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| Platforms (1) |
| GPL21785 |
Illumina HiSeq 4000 (Arabidopsis thaliana) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE122528 |
mRNA adenosine methylase (MTA) deposits m6A on pri-miRNAs to modulate miRNA biogenesis in Arabidopsis thaliana |
|
| Relations |
| BioProject |
PRJNA505486 |
| SRA |
SRP168612 |
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