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| Status |
Public on Dec 31, 2018 |
| Title |
Deep single-cell RNAseq of Gpnmb+Clec7a+ microglia from postnatal day 7 cerebellum |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We generated single-cell RNAseq profiles of 143 microglia, sorted in the gate CD45lowCD11+Gpnmb+Clec7a+, from postnatal day 7 cerebellum to validate the newly identified “proliferative region-associated microglia (PAM)” (Gpnmb and Clec7a are PAM surface markers). Single cells were FACS index sorted followed by Smart-seq2 library preparation and Illumina Nextseq (sequence depth > 1 million per cell). These cells showed characteristic PAM gene expression and clustered together with other PAM cells sequenced in the same study.
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| Overall design |
Single microglia were FACS sorted from pooled male animal samples (C57BL/6N) into 96-well plates. Libraries were prepared with a semi-automated Smart-seq2 protocol. All 143 cells passed the three QC criteria (Y=passed).
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| |
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| Contributor(s) |
Li Q, Barres B |
| Citation(s) |
30606613 |
| |
| Submission date |
Nov 27, 2018 |
| Last update date |
Apr 01, 2019 |
| Contact name |
Qingyun Li |
| E-mail(s) |
tristan.qingyun.li@gmail.com
|
| Phone |
702-686-6685
|
| Organization name |
Stanford University
|
| Department |
Neurobiology
|
| Street address |
299 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (143)
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| This SubSeries is part of SuperSeries: |
| GSE123030 |
Developmental heterogeneity of microglia and brain myeloid cells revealed by deep single-cell RNA sequencing |
|
| Relations |
| BioProject |
PRJNA507268 |
| SRA |
SRP170964 |
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