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Status |
Public on Dec 31, 2018 |
Title |
Deep single-cell RNAseq of Gpnmb+Clec7a+ microglia from postnatal day 7 cerebellum |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We generated single-cell RNAseq profiles of 143 microglia, sorted in the gate CD45lowCD11+Gpnmb+Clec7a+, from postnatal day 7 cerebellum to validate the newly identified “proliferative region-associated microglia (PAM)” (Gpnmb and Clec7a are PAM surface markers). Single cells were FACS index sorted followed by Smart-seq2 library preparation and Illumina Nextseq (sequence depth > 1 million per cell). These cells showed characteristic PAM gene expression and clustered together with other PAM cells sequenced in the same study.
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Overall design |
Single microglia were FACS sorted from pooled male animal samples (C57BL/6N) into 96-well plates. Libraries were prepared with a semi-automated Smart-seq2 protocol. All 143 cells passed the three QC criteria (Y=passed).
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Contributor(s) |
Li Q, Barres B |
Citation(s) |
30606613 |
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Submission date |
Nov 27, 2018 |
Last update date |
Apr 01, 2019 |
Contact name |
Qingyun Li |
E-mail(s) |
tristan.qingyun.li@gmail.com
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Phone |
702-686-6685
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Organization name |
Stanford University
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Department |
Neurobiology
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Street address |
299 Campus Drive
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City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305 |
Country |
USA |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (143)
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This SubSeries is part of SuperSeries: |
GSE123030 |
Developmental heterogeneity of microglia and brain myeloid cells revealed by deep single-cell RNA sequencing |
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Relations |
BioProject |
PRJNA507268 |
SRA |
SRP170964 |