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Status |
Public on Dec 08, 2008 |
Title |
Transcriptional activity of Bacillus subtilis exposed to lethal heat stress |
Organism |
Bacillus subtilis |
Experiment type |
Expression profiling by array
|
Summary |
Transcriptional profiling of Bacillus subtilis to lethal heat stress by systematic sampling of bacterial cell suspensions exposed to gradually increasing temperatures.
Keywords: Stress response
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Overall design |
Bacterial cell suspensions exposed to increasing temperatures ranging from 30-62ºC (Cy5 channel) versus 30 degrees C reference (Cy 3 channel), by transfer of the bacterial cell suspension from a 30ºC to a 65ºC water bath; sampling and quenching at 30, 40, 50, 57, 58, 59, 60 and 62ºC; total time to reach 62ºC is ~12 minutes . Biological replicates: 2, independently grown cultures. One replicate per array. Strains B subtilis 168 1A1; B subtilis A163 (food product isolate); B. subtilis MC85 (food product isolate).
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Contributor(s) |
Kort R |
Citation(s) |
19061518 |
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Submission date |
Aug 07, 2008 |
Last update date |
Mar 20, 2012 |
Contact name |
Remco Kort |
E-mail(s) |
remco.kort@tno.nl
|
Phone |
+31 30 694 4625
|
Fax |
+31 30 694 4466
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URL |
http://www.tno.nl/microbialgenomics
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Organization name |
TNO
|
Department |
Microbiology
|
Lab |
Microbial Genomics
|
Street address |
Utrechtseweg 48
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City |
Zeist |
ZIP/Postal code |
3704HE |
Country |
Netherlands |
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Platforms (1) |
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Samples (19)
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GSM310615 |
Bacillus subtilis 1A1 50ºC replicate 1 |
GSM310616 |
Bacillus subtilis 1A1 50ºC replicate 2 |
GSM310617 |
Bacillus subtilis 1A1 57ºC replicate 1 |
GSM310618 |
Bacillus subtilis 1A1 57ºC replicate 2 |
GSM310619 |
Bacillus subtilis 1A1 58ºC replicate 1 |
GSM310620 |
Bacillus subtilis 1A1 58ºC replicate 2 |
GSM310621 |
Bacillus subtilis 1A1 59ºC replicate 1 |
GSM310622 |
Bacillus subtilis 1A1 59ºC replicate 2 |
GSM310623 |
Bacillus subtilis 1A1 60ºC replicate 1 |
GSM310624 |
Bacillus subtilis 1A1 60ºC replicate 2 |
GSM310625 |
Bacillus subtilis 1A1 62ºC replicate 1 |
GSM310626 |
Bacillus subtilis 1A1 62ºC replicate 2 |
GSM310627 |
Bacillus subtilis A163 50ºC |
GSM310628 |
Bacillus subtilis A163 60ºC |
GSM310629 |
Bacillus subtilis MC85 50ºC |
GSM310630 |
Bacillus subtilis MC85 60ºC |
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Relations |
BioProject |
PRJNA113207 |