|
| Status |
Public on Dec 08, 2008 |
| Title |
Transcriptional activity of Bacillus subtilis exposed to lethal heat stress |
| Organism |
Bacillus subtilis |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptional profiling of Bacillus subtilis to lethal heat stress by systematic sampling of bacterial cell suspensions exposed to gradually increasing temperatures.
Keywords: Stress response
|
| |
|
| Overall design |
Bacterial cell suspensions exposed to increasing temperatures ranging from 30-62ºC (Cy5 channel) versus 30 degrees C reference (Cy 3 channel), by transfer of the bacterial cell suspension from a 30ºC to a 65ºC water bath; sampling and quenching at 30, 40, 50, 57, 58, 59, 60 and 62ºC; total time to reach 62ºC is ~12 minutes . Biological replicates: 2, independently grown cultures. One replicate per array. Strains B subtilis 168 1A1; B subtilis A163 (food product isolate); B. subtilis MC85 (food product isolate).
|
| |
|
| Contributor(s) |
Kort R |
| Citation(s) |
19061518 |
| |
| Submission date |
Aug 07, 2008 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Remco Kort |
| E-mail(s) |
remco.kort@tno.nl
|
| Phone |
+31 30 694 4625
|
| Fax |
+31 30 694 4466
|
| URL |
http://www.tno.nl/microbialgenomics
|
| Organization name |
TNO
|
| Department |
Microbiology
|
| Lab |
Microbial Genomics
|
| Street address |
Utrechtseweg 48
|
| City |
Zeist |
| ZIP/Postal code |
3704HE |
| Country |
Netherlands |
| |
|
| Platforms (1) |
|
| Samples (19)
|
| GSM310615 |
Bacillus subtilis 1A1 50ºC replicate 1 |
| GSM310616 |
Bacillus subtilis 1A1 50ºC replicate 2 |
| GSM310617 |
Bacillus subtilis 1A1 57ºC replicate 1 |
| GSM310618 |
Bacillus subtilis 1A1 57ºC replicate 2 |
| GSM310619 |
Bacillus subtilis 1A1 58ºC replicate 1 |
| GSM310620 |
Bacillus subtilis 1A1 58ºC replicate 2 |
| GSM310621 |
Bacillus subtilis 1A1 59ºC replicate 1 |
| GSM310622 |
Bacillus subtilis 1A1 59ºC replicate 2 |
| GSM310623 |
Bacillus subtilis 1A1 60ºC replicate 1 |
| GSM310624 |
Bacillus subtilis 1A1 60ºC replicate 2 |
| GSM310625 |
Bacillus subtilis 1A1 62ºC replicate 1 |
| GSM310626 |
Bacillus subtilis 1A1 62ºC replicate 2 |
| GSM310627 |
Bacillus subtilis A163 50ºC |
| GSM310628 |
Bacillus subtilis A163 60ºC |
| GSM310629 |
Bacillus subtilis MC85 50ºC |
| GSM310630 |
Bacillus subtilis MC85 60ºC |
|
| Relations |
| BioProject |
PRJNA113207 |
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