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| Status |
Public on May 15, 2019 |
| Title |
Taenia crassiceps excreted/secreted products alters the microRNA profile and regulates the pro-inflammatory response of M1 macrophages [mRNA] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We tested the ability of helminth Taenia crassiceps parasite excreted/secreted products (TcES) to induce a regulatory-associated profile in bone-marrow derived macrophages (BMDMs). We also compared gene expression and microRNA profile of BMDMs exposed to TcES with those exposed to LPS which elicits a well-known inflammatory program.
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| Overall design |
Mature macrophages were exposed for 4 or 24 hr to LPS (1 ug/ml), T. crassiceps excreted/secreted products (25ug/ml) or T. crassiceps excreted/secreted products plus LPS. After these time points, total RNA were collected. Next, 15ng of mRNA or microRNA from each group were hybridized overnight following manufacturer's instructions and gene expression was compared to unstimulated BMDMs.
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| Contributor(s) |
MartÃnez-Saucedo D, Terrazas CA |
| Citation(s) |
31218234 |
| Submission date |
Jan 16, 2019 |
| Last update date |
Jun 24, 2019 |
| Contact name |
Luis Ignacio Terrazas Valdes |
| E-mail(s) |
literrazas@unam.mx
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| Organization name |
UNAM
|
| Street address |
Av. de los Barrios 1, colonia Los Reyes Iztacala
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| City |
Tlalnepantla de Baz |
| State/province |
Estado de Mexico |
| ZIP/Postal code |
54090 |
| Country |
Mexico |
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| Platforms (1) |
| GPL20885 |
nCounter Mouse Inflammation panel V2 |
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| Samples (7)
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| This SubSeries is part of SuperSeries: |
| GSE125172 |
Taenia crassiceps excreted/secreted products alters the microRNA profile and regulates the pro-inflammatory response of M1 macrophages |
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| Relations |
| BioProject |
PRJNA515473 |
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