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| Status |
Public on Oct 31, 2008 |
| Title |
The growth factor environment defines distinct pluripotent ground states in novel blastocyst derived stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Pluripotent stem cell lines can be derived from blastocyst embryos, which yield embryonic stem cell lines (ES cells), as well as the post-implantation epiblast, which gives rise to epiblast stem cell lines (EpiSCs). Remarkably, ES cells and EpiSCs display profound differences in the combination of growth factors that maintain their pluripotent state. Molecular and functional differences between these two stem cell types demonstrate that the tissue of origin and/or the growth factor milieu may be important determinants of the stem cell identity. We explored how developmental stage of the tissue of origin and culture growth factor conditions affect the stem cell pluripotent state. Our findings reveal that novel stem cell lines can be generated from blastocyst embryos with unique functional and molecular properties. We demonstrate that the culture growth factor environment and cell-cell interaction play a critical role in defining several unique and stable stem cell ground states.
Keywords: Clonal analysis of FAB-SC gene expression
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| |
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| Overall design |
Cell type characterization - 3 separate single cell clones
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| |
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| Contributor(s) |
Chou Y, Chen H, Eijpe M, Yabuuchi A, Chenoweth JG, Lu J, McKay RD, Geijsen N |
| Citation(s) |
18984157 |
| |
| Submission date |
Aug 21, 2008 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Niels Geijsen |
| Organization name |
Massachusetts General Hospital
|
| Department |
Center for Regenerative Medicine
|
| Street address |
185 Cambridge St. CPZN 4-4256
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL7204 |
GeneSifter export of Agilent 4X44K whole mouse genome microarray |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA112917 |
