|
| Status |
Public on Oct 31, 2008 |
| Title |
FAB-SC2 |
| Sample type |
RNA |
| |
|
| Source name |
Clonal FAB-SC unstimulated, clone2
|
| Organism |
Mus musculus |
| Characteristics |
Clonal FAB-SC unstimulated, clone2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol(Invitrogen) following the manufacturer's protocol
|
| Label |
Cy3
|
| Label protocol |
cRNAs were amplified and labeled with Cy3 using Agilent's RNA labeling kit
|
| |
|
| Hybridization protocol |
hybridization to and the washing of the Agilent 4X44K slides was carried out following the manufacturer's protocols
|
| Scan protocol |
Scanning was done in an Agilent scanner using the manufacturer's software and protocols(GE1-v5_95_Feb07)
|
| Description |
Cells were derived under FAB-SC conditions and 3 separate single cell clones were used for gene expression analysis
|
| Data processing |
Data was processed with the GeneSifter program, normalized to median
|
| |
|
| Submission date |
Aug 21, 2008 |
| Last update date |
Aug 25, 2008 |
| Contact name |
Niels Geijsen |
| Organization name |
Massachusetts General Hospital
|
| Department |
Center for Regenerative Medicine
|
| Street address |
185 Cambridge St. CPZN 4-4256
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL7204 |
| Series (1) |
| GSE12519 |
The growth factor environment defines distinct pluripotent ground states in novel blastocyst derived stem cells |
|
