GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE125755 Query DataSets for GSE125755
Status Public on Jan 24, 2022
Title Copper regulation of HIF-1 transcriptional activities
Organism Macaca mulatta
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary HIF-1 regulated VEGF expression were upregulated in reponse to ischemic injury. However, the VEGF mRNA level were increased in the early stage but decreased in the late stage of myocardial infarction. We found that activities of HIF-1 binding to the HRE sites were possiblely responsible for the differential expression of VEGF. The HIF-1 binding activities were regulated by copper. After ischemic injury, copper concentrations were decreased in the infarct myocardium in the late stage of myocardial infarction, which might reduce VEGF expression through insufficient HIF-1 binding to HRE sites.
Overall design Rhesus monkeys were subjected to LAD ligation to induce myocardial infarction. After 6 hours or 4 weeks, the hearts were collected and the infarct myocardium were separated to detect VEGF expression by qRT-PCR and to measure HIF-1 binding acitivies by ChIP-Seq. Immunohistochestry was applied for HIF-1α detection. Copper concentrations were determined by atomic absorption spectrometry.
Contributor(s) Zhang W, Han P
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jan 28, 2019
Last update date Jan 25, 2022
Contact name Wenjing Zhang
Phone (001)9015030686
Organization name West China Hospital,Sichuan University
Department Regenerative Medicine Research Center
Street address Keyuan Road 4,Gaopeng Street
City Chengdu
State/province Sichuan
ZIP/Postal code 610041
Country China
Platforms (1)
GPL24522 HiSeq X Ten (Macaca mulatta)
Samples (4)
GSM3581500 MI 6 h
GSM3581501 MI 4 w
GSM3581502 InPut 6h
BioProject PRJNA517453
SRA SRP182674

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125755_RAW.tar 190.0 Kb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap