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| Status |
Public on Sep 26, 2019 |
| Title |
Loss of Bap1 leads to an increased global methylation and decrease in H3K27AC enhancer marks around key lineage commitment genes in Xenopus laevis embryos [ChIP-seq] |
| Organism |
Xenopus laevis |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We performed morpholino-mediated knockdown of Bap1 protein expression in Xenopus laevis developing embryos, and analyzed inhibiting and activating histone marks . We find that inhibition of Bap1 leads to decrease in H3K27AC activating makrs around lineage commitment genes, and increased global methylation.
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| Overall design |
Control and Bap1-depleted stage 12 frog embryos were analyzed by ChIP-seq for expression of H3K27AC, H3K27me3, H2Aub and H3K4me3 histone marks.
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| Contributor(s) |
Kuznetsov JN, Kurtenbach S |
| Citation(s) |
31555735 |
| Submission date |
Feb 14, 2019 |
| Last update date |
Oct 01, 2019 |
| Contact name |
Michael Durante |
| E-mail(s) |
mdurante@med.miami.edu
|
| Phone |
2393575338
|
| Organization name |
University of Miami Miller School of Medicine
|
| Department |
Ophthalmology
|
| Lab |
Harbour
|
| Street address |
1501 NW 10th Ave., Ste 832
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
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| Platforms (1) |
| GPL21248 |
Illumina NextSeq 500 (Xenopus laevis) |
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| Samples (20)
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| This SubSeries is part of SuperSeries: |
| GSE126599 |
Loss of Bap1 in Xenopus laevis embryos |
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| Relations |
| BioProject |
PRJNA522474 |
| SRA |
SRP185948 |
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