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| Status |
Public on Apr 27, 2020 |
| Title |
Identification of herpes simplex virus 1 and host targets of miR-138 by comparative PAR-CLIP in Neuro-2a and 293T cells |
| Organisms |
Homo sapiens; Mus musculus; Human alphaherpesvirus 1 |
| Experiment type |
Other
|
| Summary |
This is a part of the study that shows that a host microRNA, miR-138, represses herpes simplex virus 1 (HSV-1) gene expression through both viral and host targets. These PAR-CLIP analyses identified viral and host targets of miR-138 in Neuro-2a (mouse neuroblastoma) and 293T (human embryonic kidney) cells. We constructed two cell lines derived from Neuro-2a cells, one overexpressing miR-138 (N2A138) and one antagonizing miR-138 (N2Aanti138). We also constructed two cell lines derived from 293T cells, one overexpressing miR-138 (293T138) and one control cells (293Tcontrol). Uninfected N2A138 and N2Aanti138 were compared by PAR-CLIP for host targets in Neuro-2A cells. 293T138 and 293Tcontrol cells infected for 4 and 8 hours were compared by PAR-CLIP for HSV-1 targets in 293T cells. 293T138 and 293Tcontrol cells infected for 4 hours were also compared for host targets in 293T cells.
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| Overall design |
Comparison of cells overexpressing miR-138 with control cells. Infect the cells for viral target identification
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| Contributor(s) |
Pan D, Coen DM |
| Citation(s) |
33558653, 38443365 |
| |
| Submission date |
Feb 28, 2019 |
| Last update date |
Mar 13, 2024 |
| Contact name |
Dongli Pan |
| E-mail(s) |
pandongli@zju.edu.cn
|
| Organization name |
Zhejiang University
|
| Street address |
866 Yuhangtang Road
|
| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
110058 |
| Country |
China |
| |
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| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL26236 |
Illumina NextSeq 500 (Homo sapiens; Human alphaherpesvirus 1) |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE127504 |
Identification of herpes simplex virus 1 and host targets of miR-138 by combined RNAseq/PARCLIP |
|
| Relations |
| BioProject |
PRJNA524884 |
| SRA |
SRP187089 |
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