|
| Status |
Public on Mar 02, 2022 |
| Title |
Mutant GTF2I induces cell transformation and metabolic alterations in thymic epithelial cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The pathogenesis of thymic epithelial tumors (TETs) is poorly understood. Recently we reported the frequent occurrence of a missense mutation in the GTF2I gene in TETs and hypothesized that GTF2I mutation might contribute to thymic tumorigenesis. Expression of mutant TFII-I altered the transcriptome of normal thymic epithelial cells and upregulated several oncogenic genes. Gtf2i L424H knockin cells exhibited cell transformation, aneuploidy, and increase tumor growth and survival under glucose deprivation or DNA damage. Gtf2i mutation also increased the expression of several glycolytic enzymes, cyclooxygenase-2, and caused modifications of lipid metabolism. Elevated cyclooxygenase-2 expression by Gtf2i mutation was required for survival under metabolic stress and cellular transformation of thymic epithelial cells. Our findings identify GTF2I mutation as a new oncogenic driver that is responsible for transformation of thymic epithelial cells.
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| |
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| Overall design |
Refer to individual Series
|
| Web link |
https://pubmed.ncbi.nlm.nih.gov/32034314/
|
| |
|
| Citation(s) |
32034314 |
| |
| Submission date |
Mar 06, 2019 |
| Last update date |
Mar 03, 2022 |
| Contact name |
IN-KYU KIM |
| E-mail(s) |
ingyu.kim80@gmail.com
|
| Phone |
202-687-4738
|
| Organization name |
Georgetown University Medical Center
|
| Department |
Oncology
|
| Lab |
Dr. Giaccone's
|
| Street address |
3970 RESERVOIR RD NW, New Research Bldg E212
|
| City |
WASHINTON |
| State/province |
DC |
| ZIP/Postal code |
20057 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
|
| Samples (16)
|
| GSM3656375 |
TEC100.4/TFII-I-βmt_Doxycycline+ (GE1_1_1) |
| GSM3656376 |
TEC100.4/TFII-I-βmt_Doxycycline+ (GE1_1_2) |
| GSM3656377 |
TEC100.4/TFII-I-δmt_Doxycycline+ (GE1_1_3) |
|
| This SuperSeries is composed of the following SubSeries: |
| GSE127907 |
GTF2I mutation induces metabolic alterations and cell transformation in thymic epithelial cells [I] |
| GSE127908 |
GTF2I mutation induces metabolic alterations and cell transformation in thymic epithelial cells [II] |
|
| Relations |
| BioProject |
PRJNA525777 |
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