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| Status |
Public on Oct 01, 2019 |
| Title |
Effect of cycloheximide in the co-translation mRNA degradation pattern in set2D and rrp6D strains |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We investigated cryptic transcription start site usage, chromatin organization and post-transcriptional consequences in Saccharomyces cerevisiae. We used 5PSeq approach, which measures ribosome dynamics by sequencing the presence of co-translation mRNA degradation intermediates, to assess if cryptic transcripts are engaged in active translation. Here we assay the effect of Cycloheximide treatment (CHX) in the co-translational degradation profile of using strains where cryptic transcription is enhanced. We study the profiles in set2D and rrp6D, enhanced for the presence of chromatin-sensitive and RNA degradation-sensitive cryptic transcripts respectively.
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| Overall design |
We performed 2 biologically independent 5PSeq experiments. All cells were grown in YPD. CHX treated samples were incubated for 10 min and flash frozen in N2.
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| Contributor(s) |
Sanchez YP, Zhang Y, Wang J, Pelechano V |
| Citation(s) |
31740578 |
| |
| Submission date |
Mar 20, 2019 |
| Last update date |
Jan 02, 2020 |
| Contact name |
Vicent Pelechano |
| E-mail(s) |
vicente.pelechano.garcia@ki.se
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| Organization name |
ScilifeLab - Karolinska Institutet
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| Department |
MTC
|
| Street address |
Nobels väg 16
|
| City |
Solna |
| ZIP/Postal code |
SE-17177 |
| Country |
Sweden |
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| Platforms (1) |
| GPL19756 |
Illumina NextSeq 500 (Saccharomyces cerevisiae) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA528276 |
| SRA |
SRP188964 |
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