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| Status |
Public on Dec 01, 2019 |
| Title |
The zinc finger protein CLAMP promotes long-range chromatin interactions that mediate dosage compensation of the Drosophila male X-chromosome [Hi-C seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
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| Summary |
Hi-C and 4C-seq anlaysis of Drosophila S2 cells
Drosophila dosage compensation is an important model system for defining how active chromatin domains are formed. The Male-specific lethal dosage compensation complex (MSLc) increases transcript levels of genes along the length of the single male X-chromosome to equalize with that on the two female X-chromosomes. The strongest binding sites for MSLc cluster together in three-dimensional space independent of MSLc because clustering occurs in both sexes. CLAMP, a non-sex specific, ubiquitous zinc finger protein, binds synergistically with MSLc to enrich the occupancy of both factors on the male X-chromosome. Here, we demonstrate that CLAMP promotes the observed clustering of MSLc bindings sites. Genome-wide, CLAMP promotes interactions between active chromatin regions and represses interactions between inactive chromatin regions. Moreover, the X-enriched CLAMP protein promotes longer-range interactions on the active X-chromosome than autosomes. Overall, we define how long-range interactions, mediated by a locally-enriched ubiquitous transcription factor, generate a three-dimensional active chromatin domain.
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| Overall design |
Biological duplicated Hi-C experiments of Drosophila S2 cells after RNAi of gfp (control) or clamp.
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| Contributor(s) |
Jordan W, Larschan E |
| Citation missing |
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| |
| Submission date |
May 01, 2019 |
| Last update date |
Dec 03, 2019 |
| Contact name |
Erica Larschan |
| E-mail(s) |
Erica_Larschan@brown.edu
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| Organization name |
Brown University
|
| Street address |
185 Meeting St.
|
| City |
Providence |
| ZIP/Postal code |
02912 |
| Country |
USA |
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| Platforms (1) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE130546 |
The zinc finger protein CLAMP promotes long-range chromatin interactions that mediate dosage compensation of the Drosophila male X-chromosome |
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| Relations |
| BioProject |
PRJNA540595 |
| SRA |
SRP194366 |
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