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| Status |
Public on May 31, 2019 |
| Title |
Gene expression changes in astrocytes by pulsed radiofrequency: a possible mechanism of neuropathic pain improvement |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We investigated genomic effects of pulsed radiofrequency (PRF) on mouse cultured astrocyte cell line, C8D1A cells, using microarray analysis. PRF increased 2,431 genes and decreased 209 genes. Among these genes, 435 genes were upregulated with more than 10-fold, 89 genes with more than 30-fold, while there was no gene with 10-fold decrease. From the gene ontology analysis using the list of 10-fold upregulated genes, PRF treatment activated immune response. From pathway analysis using Kyoto Encyclopedia of Gene and Genomes using the same list, 7 pathways related with neuropathic pain were detected.
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| Overall design |
Pulsed radiofrequency-induced gene expression in cultured mouse astrocyte cell line (C8D1A) was measured and copared with non-treated cells. Three independent experiments were performed.
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| Contributor(s) |
Tanabe K, Takashima S, Iida H |
| Citation(s) |
33259929 |
| Submission date |
May 06, 2019 |
| Last update date |
Jan 09, 2021 |
| Contact name |
Kumiko Tanabe |
| E-mail(s) |
kumiko-t@m2.gyao.ne.jp
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| Organization name |
Gifu University School of Medicine
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| Street address |
Yanagido 1-1
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| City |
Gifu |
| ZIP/Postal code |
501-1194 |
| Country |
Japan |
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| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA541287 |
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