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| Status |
Public on Dec 31, 2019 |
| Title |
Gene expression profiles between miRNA Negative control-treated and miR-124a-3p mimic treated-teratomas |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The role of microRNAs (miRNAs) during mouse early development, especially in endoderm germ layer formation, is largely unknown. Here, using miRNA profiling, we discovered that miR-124a negatively regulates endoderm lineage commitment in mouse embryonic stem cells (mESCs). We showed that miR-124a inhibits endoderm differentiation in vitro through targeting the 3’ untranslated region (UTR) of Sox17 and Gata6, revealing the existence of an interplay between miR-124a and Sox17/Gata6 transcription factors in hepato-specific gene regulation. Besides, we proposed an in vivo system that utilizes teratoma to evaluate the functional role of miRNA in lineage specification. We demonstrated that ectopic expression of miR-124a in teratomas suppressed endoderm and mesoderm lineage differentiation while augmented the differentiation of ectoderm lineage. Collectively, our findings suggested that miR-124a plays a significant role in mESCs lineage commitment.
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| Overall design |
Formation of teratomas derived from mESCs was performed in immunodeficiency mouse (BALB/c Nude). Mice were randomly divided into 2 groups (miR-124a vs Control) for in vivo miRNA transfection experiment (n=3 in each group). In vivo miRNA transfection was performed by intratumoral administration of miRNAs: “Atelogen QG” complexes at a final concentration of 15 µg/100 µL. In vivo delivery of miRNAs was performed once every 3 days until Day 28. At the end of the experiment, teratomas were harvested for histological analysis and microarray analysis.
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| Contributor(s) |
Liew LC, Ochiya T |
| Citation(s) |
31828873 |
| |
| Submission date |
May 16, 2019 |
| Last update date |
May 03, 2020 |
| Contact name |
Yusuke Yamamoto |
| E-mail(s) |
yuyamamo@ncc.go.jp
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| Phone |
+81-335422511
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| Organization name |
National cancer center research institute
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| Lab |
Cellular signaling
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| Street address |
5-1-1, Tsukiji, Chuo-ku
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| City |
Chuo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
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| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA543311 |
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