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| Status |
Public on Oct 16, 2008 |
| Title |
Gene expression profiling in Daphnia magna, Part III: Uncovering Biomarkers for Metal and ORC exposures |
| Organism |
Daphnia magna |
| Experiment type |
Expression profiling by array
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| Summary |
To determine toxicant specific effects of Ordnance Related Compound (ORC) exposure we performed microarray hybridizations with RNA isolated from Daphnia magna following different ORC exposures at the 1/10 LC50. The gene expression profiles revealed toxicant specific gene expression profiles allowed for the identification of specific biomarkers of exposure.
Keywords: ecotoxicogenomic exposure study
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| Overall design |
We exposed Daphnia magna the 1/10 LC50 of different Ordnance Related Compounds (Cu, Zn, Pb, WO4, RDX, TNT, 2-ADNT, 2-ADNT, TNB, DNB, 2,4-DNT, and 2,6-DNT) for 24 hours. For each exposure condition, we performed 3 exposures and 2 technical replicates (as dye swap) for each exposure (6 microarrays total, except TNT and Cu). All exposures were compared to a unexposed laboratory control (MHRW media).
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| Contributor(s) |
Garcia-Reyero N, Poynton HC, Kennedy AJ, Guan X, Escalon LL, Chang B, Varshavsky J, Loguinov AV, Vulpe CD, Perkins EJ |
| Citation(s) |
21744839 |
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| Submission date |
Oct 13, 2008 |
| Last update date |
Mar 18, 2015 |
| Contact name |
Helen C Poynton |
| E-mail(s) |
helen.poynton@umb.edu
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| Phone |
617-287-7323
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| Organization name |
UMass Boston
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| Department |
School for the Environment
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| Lab |
Poynton Lab
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| Street address |
100 Morrissey Blvd.
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02125 |
| Country |
USA |
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| Platforms (2) |
| GPL3710 |
Daphnia magna DGC cDNA array ver.1 |
| GPL5129 |
Daphnia magna cDNA microarray ver. 2 |
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| Samples (70)
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| GSM332971 |
Daphnia_1/10LC50_RDX_4 |
| GSM332972 |
Daphnia_1/10LC50_RDX_5 |
| GSM332973 |
Daphnia_1/10LC50_RDX_6 |
| GSM332974 |
Daphnia_1/10LC50_TNT_1 |
| GSM332975 |
Daphnia_1/10LC50_TNT_2 |
| GSM332976 |
Daphnia_1/10LC50_TNT_3 |
| GSM332977 |
Daphnia_1/10LC50_TNT_4 |
| GSM332978 |
Daphnia_1/10LC50_TNT_5 |
| GSM332979 |
Daphnia_1/10LC50_2ADNT_1 |
| GSM332980 |
Daphnia_1/10LC50_2ADNT_2 |
| GSM332981 |
Daphnia_1/10LC50_2ADNT_3 |
| GSM332982 |
Daphnia_1/10LC50_2ADNT_4 |
| GSM332983 |
Daphnia_1/10LC50_2ADNT_5 |
| GSM332984 |
Daphnia_1/10LC50_2ADNT_6 |
| GSM332985 |
Daphnia_1/10LC50_4ADNT_1 |
| GSM332986 |
Daphnia_1/10LC50_4ADNT_2 |
| GSM332987 |
Daphnia_1/10LC50_4ADNT_3 |
| GSM332988 |
Daphnia_1/10LC50_4ADNT_4 |
| GSM332989 |
Daphnia_1/10LC50_4ADNT_5 |
| GSM332990 |
Daphnia_1/10LC50_4ADNT_6 |
| GSM333389 |
Daphnia_1/10LC50_2,4-DNT_exp1,rep1 |
| GSM333390 |
Daphnia_1/10LC50_2,4-DNT_exp1,rep2 |
| GSM333391 |
Daphnia_1/10LC50_2,4-DNT_exp2,rep1 |
| GSM333392 |
Daphnia_1/10LC50_2,4-DNT_exp2,rep2 |
| GSM333393 |
Daphnia_1/10LC50_2,4-DNT_exp3,rep1 |
| GSM333394 |
Daphnia_1/10LC50_2,4-DNT_exp3,rep2 |
| GSM333395 |
Daphnia_1/10LC50_2,6-DNT_exp1,rep1 |
| GSM333396 |
Daphnia_1/10LC50_2,6-DNT_exp1,rep2 |
| GSM333397 |
Daphnia_1/10LC50_2,6-DNT_exp2,rep1 |
| GSM333398 |
Daphnia_1/10LC50_2,6-DNT_exp2,rep2 |
| GSM333399 |
Daphnia_1/10LC50_2,6-DNT_exp3,rep1 |
| GSM333400 |
Daphnia_1/10LC50_2,6-DNT_exp3,rep2 |
| GSM333401 |
Daphnia_1/10LC50_TNB_exp1,rep1 |
| GSM333402 |
Daphnia_1/10LC50_TNB_exp1,rep2 |
| GSM333403 |
Daphnia_1/10LC50_TNB_exp2,rep1 |
| GSM333404 |
Daphnia_1/10LC50_TNB_exp2,rep2 |
| GSM333405 |
Daphnia_1/10LC50_TNB_exp3,rep1 |
| GSM333406 |
Daphnia_1/10LC50_TNB_exp3,rep2 |
| GSM333407 |
Daphnia_1/10LC50_DNB_exp1,rep1 |
| GSM333408 |
Daphnia_1/10LC50_DNB_exp1,rep2 |
| GSM333409 |
Daphnia_1/10LC50_DNB_exp2,rep1 |
| GSM333410 |
Daphnia_1/10LC50_DNB_exp2,rep2 |
| GSM333411 |
Daphnia_1/10LC50_DNB_exp3,rep1 |
| GSM333412 |
Daphnia_1/10LC50_DNB_exp3,rep2 |
| GSM333413 |
Daphnia_1/10LC50_Cu_exp1,rep1 |
| GSM333414 |
Daphnia_1/10LC50_Cu_exp1,rep2 |
| GSM333415 |
Daphnia_1/10LC50_Cu_exp2,rep1 |
| GSM333416 |
Daphnia_1/10LC50_Cu_exp2,rep2 |
| GSM333417 |
Daphnia_1/10LC50_Cu_exp3,rep2 |
| GSM333418 |
Daphnia_1/10LC50_Zn_exp1,rep1 |
| GSM333419 |
Daphnia_1/10LC50_Zn_exp1,rep2 |
| GSM333420 |
Daphnia_1/10LC50_Zn_exp2,rep1 |
| GSM333421 |
Daphnia_1/10LC50_Zn_exp2,rep2 |
| GSM333422 |
Daphnia_1/10LC50_Zn_exp3,rep1 |
| GSM333423 |
Daphnia_1/10LC50_Zn_exp3,rep2 |
| GSM333424 |
Daphnia_1/10LC50_Pb_exp1,rep1 |
| GSM333425 |
Daphnia_1/10LC50_Pb_exp1,rep2 |
| GSM333426 |
Daphnia_1/10LC50_Pb_exp2,rep1 |
| GSM333427 |
Daphnia_1/10LC50_Pb_exp2,rep2 |
| GSM333428 |
Daphnia_1/10LC50_Pb_exp3,rep1 |
| GSM333429 |
Daphnia_1/10LC50_Pb_exp3,rep2 |
| GSM333430 |
Daphnia_1/10LC50_WO4_exp1,rep1 |
| GSM333431 |
Daphnia_1/10LC50_WO4_exp1,rep2 |
| GSM333432 |
Daphnia_1/10LC50_WO4_exp2,rep1 |
| GSM333433 |
Daphnia_1/10LC50_WO4_exp2,rep2 |
| GSM333434 |
Daphnia_1/10LC50_WO4_exp3,rep1 |
| GSM333435 |
Daphnia_1/10LC50_WO4_exp3,rep2 |
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| Relations |
| BioProject |
PRJNA109639 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE13169_RAW.tar |
39.9 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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