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Series GSE13169 Query DataSets for GSE13169
Status Public on Oct 16, 2008
Title Gene expression profiling in Daphnia magna, Part III: Uncovering Biomarkers for Metal and ORC exposures
Organism Daphnia magna
Experiment type Expression profiling by array
Summary To determine toxicant specific effects of Ordnance Related Compound (ORC) exposure we performed microarray hybridizations with RNA isolated from Daphnia magna following different ORC exposures at the 1/10 LC50. The gene expression profiles revealed toxicant specific gene expression profiles allowed for the identification of specific biomarkers of exposure.

Keywords: ecotoxicogenomic exposure study
 
Overall design We exposed Daphnia magna the 1/10 LC50 of different Ordnance Related Compounds (Cu, Zn, Pb, WO4, RDX, TNT, 2-ADNT, 2-ADNT, TNB, DNB, 2,4-DNT, and 2,6-DNT) for 24 hours. For each exposure condition, we performed 3 exposures and 2 technical replicates (as dye swap) for each exposure (6 microarrays total, except TNT and Cu). All exposures were compared to a unexposed laboratory control (MHRW media).
 
Contributor(s) Garcia-Reyero N, Poynton HC, Kennedy AJ, Guan X, Escalon LL, Chang B, Varshavsky J, Loguinov AV, Vulpe CD, Perkins EJ
Citation(s) 21744839
Submission date Oct 13, 2008
Last update date Mar 18, 2015
Contact name Helen C Poynton
E-mail(s) helen.poynton@umb.edu
Phone 617-287-7323
Organization name UMass Boston
Department School for the Environment
Lab Poynton Lab
Street address 100 Morrissey Blvd.
City Boston
State/province MA
ZIP/Postal code 02125
Country USA
 
Platforms (2)
GPL3710 Daphnia magna DGC cDNA array ver.1
GPL5129 Daphnia magna cDNA microarray ver. 2
Samples (70)
GSM332968 Daphnia_1/10LC50_RDX_1
GSM332969 Daphnia_1/10LC50_RDX_2
GSM332970 Daphnia_1/10LC50_RDX_3
Relations
BioProject PRJNA109639

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13169_RAW.tar 39.9 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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