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Status
Public on Dec 22, 2006
Title
Daphnia magna DGC cDNA array ver.1
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Daphnia magna Manufacturer
UC Berkeley Dept. of Nutr. Sci. and Toxicology Genomics Facility
Manufacture protocol
The 5000 randomly selected cDNA clones from the Daphnia Genome Consortium (DGC) library (generous gift from D. Bauer at University of New Hampshire, NH and J. Colbourne at Indiana University, IN) were PCR amplified from the pDNR-LIB vector using the following primers: forward: AGTCGACGGTACCGGACATA and reverse: GCCAAACGAATGGTCTAGAAA. PCR products were purified by ethanol precipitation and resuspended in distilled water. cDNA clones were printed onto lysine-coated glass slides (Cheung et al. 1999).
Support
glass
Coating
polysine
Contributor(s)
Holman PS , Poynton HC , Bauer DJ , Colbourne JK Submission date
Apr 29, 2006
Last update date
Dec 22, 2006
Contact name
Helen C Poynton
E-mail(s)
helen.poynton@umb.edu Phone
617-287-7323
Organization name
UMass Boston
Department
School for the Environment
Lab
Poynton Lab
Street address
100 Morrissey Blvd.
City
Boston
State/province
MA
ZIP/Postal code
02125
Country
USA
Samples (83)
GSM107567 , GSM107581 , GSM107582 , GSM107583 , GSM107584 , GSM107585
GSM107588 ,
GSM107590 ,
GSM107592 ,
GSM107593 ,
GSM107594 ,
GSM107595 ,
GSM107596 ,
GSM107597 ,
GSM107598 ,
GSM107599 ,
GSM107600 ,
GSM107601 ,
GSM185769 ,
GSM185770 ,
GSM185771 ,
GSM185772 ,
GSM185773 ,
GSM185774 ,
GSM185775 ,
GSM185776 ,
GSM185777 ,
GSM185778 ,
GSM185779 ,
GSM185780 ,
GSM185781 ,
GSM185782 ,
GSM185783 ,
GSM185784 ,
GSM185785 ,
GSM185786 ,
GSM185787 ,
GSM185788 ,
GSM185789 ,
GSM185790 ,
GSM185791 ,
GSM185792 ,
GSM185793 ,
GSM185794 ,
GSM185795 ,
GSM185796 ,
GSM185797 ,
GSM185798 ,
GSM185799 ,
GSM185800 ,
GSM185801 ,
GSM185802 ,
GSM185803 ,
GSM185804 ,
GSM185805 ,
GSM185806 ,
GSM185807 ,
GSM185808 ,
GSM185809 ,
GSM185810 ,
GSM332968 ,
GSM332969 ,
GSM332970 ,
GSM332971 ,
GSM332972 ,
GSM332973 ,
GSM332974 ,
GSM332975 ,
GSM332976 ,
GSM332977 ,
GSM332978 ,
GSM332979 ,
GSM332980 ,
GSM332981 ,
GSM332982 ,
GSM332983 ,
GSM332984 ,
GSM332985 ,
GSM332986 ,
GSM332987 ,
GSM332988 ,
GSM332989 ,
GSM332990 Series (3)
GSE4759 1/10 LC50 metal exposures to Daphnia magna
GSE7668 Concentration dependent gene expression changes in Daphnia magna exposed to metals
GSE13169 Gene expression profiling in Daphnia magna, Part III: Uncovering Biomarkers for Metal and ORC exposures
Data table header descriptions ID Plate_ID cDNA library plate where clone can be found
Well_ID Well where clone can be found
CLONE ID GB_ACC Genbank accession
SPOT_ID
Data table ID Plate_ID Well_ID CLONE ID GB_ACC SPOT_ID 1
P1A
A1
none
unknown
2
P1A
A3
none
unknown
3
P1A
A5
none
unknown
4
P1A
A7
none
unknown
5
P1A
A9
none
unknown
6
P1A
A11
none
unknown
7
P1A
C1
none
unknown
8
P1A
C3
none
unknown
9
P1A
C5
none
unknown
10
P1A
C7
none
unknown
11
P1A
C9
none
unknown
12
P1A
C11
none
unknown
13
P1A
E1
none
unknown
14
P1A
E3
none
unknown
15
P1A
E5
none
unknown
16
P1A
E7
none
unknown
17
P1A
E9
none
unknown
18
P1A
E11
none
unknown
19
P1A
G1
none
unknown
20
P1A
G3
none
unknown
4992 129 Kbytes .Supplementary data files not provided
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