NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE133692 Query DataSets for GSE133692
Status Public on Jul 21, 2019
Title Genome-wide CRISPR screening identifies new regulators of glycoprotein secretion
Organism Homo sapiens
Experiment type Other
Summary Background: The fundamental process of protein secretion from eukaryotic cells has been well described for many years, yet gaps in our understanding of how this process is regulated remain.
Methods: With the aim of identifying novel genes involved in the secretion of glycoproteins, we used a screening pipeline consisting of a pooled genome-wide CRISPR screen, followed by secondary siRNA screening of the hits to identify and validate several novel regulators of protein secretion.
Results: We present approximately 50 novel genes not previously associated with protein secretion, many of which also had an effect on the structure of the Golgi apparatus. We further studied a small selection of hits to investigate their subcellular localization. One of these, GPR161, is a novel Golgi-resident protein that we propose maintains Golgi structure via an interaction with golgin A5.
Conclusions: This study has identified new factors for protein secretion involved in Golgi homeostasis.
 
Overall design Genome-wide CRISPR screening
Web link https://doi.org/10.12688/wellcomeopenres.15232.2
 
Contributor(s) Popa SJ, Villeneuve J, Stewart S, Garcia EP, Harrison AP, Moreau K
Citation(s) 32030357
Submission date Jul 02, 2019
Last update date Feb 10, 2020
Contact name Brian Yee Hong Lam
E-mail(s) yhbl2@cam.ac.uk
Organization name University of Cambridge
Department Metabolic Research Laboratories
Street address Box 289 Addenbookes Hospital, Hills Road
City Cambridge
ZIP/Postal code CB2 0QQ
Country United Kingdom
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (60)
GSM3916320 Suspension HeLa-Cas9 cells, N1_0a_03
GSM3916321 Suspension HeLa-Cas9 cells, N1_0a_15
GSM3916322 Suspension HeLa-Cas9 cells, N1_0a_27
Relations
BioProject PRJNA552266
SRA SRP212759

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE133692_S1.xlsx 8.5 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap