|
| Status |
Public on Jul 21, 2019 |
| Title |
Suspension HeLa-Cas9 cells, N2_8a_29 |
| Sample type |
SRA |
| |
|
| Source name |
Suspension HeLa-Cas9 cells transfected with Brunello library
|
| Organism |
Homo sapiens |
| Characteristics |
sort id: Sort 2, negative
library description: Galectin-3 negative population after sort 2 (lowest 2 %)
library: N2_a
time: 8 days post sort
collection replicate: 1
pcr replicate: 3
|
| Treatment protocol |
Hela-Cas9 cells were transfected with Brunello library
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA purification using Gentra Puregene cell kit, 1-2 x 10^7 protocol
Crispr Screening via PCR of sgRNAs according to protocol by Doench et al., DOI: 10.1038/nbt.3437
Crispr Library DNA Sequencing
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
DNA from Suspension HeLa-Cas9 cells transfected with Brunello library N2_8a_29-N2_8a_29
|
| Data processing |
Library strategy: CRISPR screen
The fastq files were combined based on PCR replicates and were processed using MaGECK to generate sgRNA counts. Brunello sgRNA library was used as the reference for counting
Supplementary_files_format_and_content: Count data table and the reference Brunello sgRNA Library provided in MS Excel format
|
| |
|
| Submission date |
Jul 02, 2019 |
| Last update date |
Jul 21, 2019 |
| Contact name |
Brian Yee Hong Lam |
| E-mail(s) |
yhbl2@cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Metabolic Research Laboratories
|
| Street address |
Box 289 Addenbookes Hospital, Hills Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0QQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL20301 |
| Series (1) |
| GSE133692 |
Genome-wide CRISPR screening identifies new regulators of glycoprotein secretion |
|
| Relations |
| BioSample |
SAMN12185320 |
| SRA |
SRX6387572 |
