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Series GSE134345 Query DataSets for GSE134345
Status Public on Aug 27, 2019
Title Transcriptomic analysis reveals potential mechanisms of toxicity in a combined exposure to dibutyl phthalate and diisobutyl phthalate in zebrafish (Danio rerio) ovary
Organism Danio rerio
Experiment type Expression profiling by high throughput sequencing
Summary Phthalate esters (PAEs), a notable plasticizer, could be prolific contaminants in the aquatic environment, and have been shown to induce reproductive toxicity. However, studies concerning the toxicity towards aquatic species are based upon individual chemicals and the combined toxicity of PAEs to aquatic organisms remains unclear. The aim of this study was to explore the potential toxic mechanism of combined exposure to dibutyl phthalate (DBP) and diisobutyl phthalate (DiBP) in adult female zebrafish ovarian. Zebrafish were exposed to DBP, DiBP and their mixtures for 30 days, and their effects on ovarian histology, plasma sex hormones and ovarian transcriptomics were investigated. The plasma estradiol (E2) levels were significantly decreased 38.9% for DBP-1133 exposure group and 41.0% for DiBP-1038 exposure group. The percentages of late/mature oocyte were also significantly decreased 17.3% in DBP-1133 exposure and 16.2% in DiBP-1038 exposure, while those in combined exposure were not significantly affected. Nonetheless, transcriptome sequencing discovered 2564 differential expressed genes (DEGs) in zebrafish ovary after exposure to the mixtures. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis identified that those DEGs were involved in the neuroactive ligand-receptor interaction, GnRH, progesterone-mediated oocyte maturation, oocyte meiosis and steroid hormone biosynthesis signaling pathways. These results revealed that combined exposure showed potential reproductive toxicity at the molecular level.
 
Overall design Ovarian mRNA sequencing of adult female zebrafish after single and combined exposure to DBP and DiBP for 30 days
 
Contributor(s) Chen H, Feng W, Chen K, Qiu X, Xu H, Mao G, Zhao T, Ding Y, Wu X
Citation(s)
  • Chen H, Feng W, Chen K, Qiu X et al. Transcriptomic analysis reveals potential mechanisms of toxicity in a combined exposure to dibutyl phthalate and diisobutyl phthalate in zebrafish (Danio rerio) ovary. Aquat Toxicol 2019 Nov;216:105290. PMID: 31518775
Submission date Jul 16, 2019
Last update date Nov 26, 2019
Contact name Hui Chen
E-mail(s) chenhui_612@126.com
Organization name jiangsu university
Street address Xuefu Road
City Zhenjiang
ZIP/Postal code 212013
Country China
 
Platforms (1)
GPL23085 HiSeq X Ten (Danio rerio)
Samples (4)
GSM3942696 CK1 (Control)
GSM3942697 B1 (DBP)
GSM3942698 I1 (DiBP)
Relations
BioProject PRJNA554813
SRA SRP214796

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE134345_RAW.tar 1.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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