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| Status |
Public on Dec 11, 2019 |
| Title |
Regulated local synthesis of ribosomal proteins maintains ribosome function in axons |
| Organism |
Xenopus laevis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Ribosome assembly occurs mainly in the nucleolus yet recent studies have revealed robust enrichment and translation of mRNAs encoding many ribosomal proteins (RPs) in axons, far away from neuronal cell bodies. Here, we report a physical and functional interaction between locally synthesized RPs and ribosomes in the axon. We show that axonal RP translation is regulated through a novel sequence motif, CUIC, that forms an RNA-loop structure in the region immediately upstream of the initiation codon. Using imaging and subcellular proteomics techniques, we show that RPs synthesized in axons join axonal ribosomes in a nucleolus-independent fashion. Inhibition of axonal CUIC-regulated RP translation causes a significant decline in local translation activity and markedly reduces axon branching in the brain, revealing the physiological relevance of axonal RP synthesis in vivo. These results suggest that axonal translation supplies cytoplasmic RPs to maintain/modify local ribosomal function far from the nucleolus in neurons.
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| Overall design |
The profiling of mRNAs in Xenopus laevis retinal ganglion cell axons
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| Contributor(s) |
Shigeoka T, Koppers M, Wong HH, Lin JQ, Cagnetta R, Dwivedy A, de Freitas Nascimento J, van Tartwijk F, Ströhl F, Cioni J, Schaeffer J, Carrington M, Kaminski CF, Jung H, Harris WA, Holt CE |
| Citation(s) |
31825839 |
| Submission date |
Aug 07, 2019 |
| Last update date |
Dec 31, 2019 |
| Contact name |
Christine E Holt |
| Organization name |
University of Cambridge
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| Department |
Department of PDN
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| Street address |
Anatomy Bldg., Downing St.
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| City |
Cambridge |
| ZIP/Postal code |
CB2 3DY |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL21248 |
Illumina NextSeq 500 (Xenopus laevis) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA559103 |
| SRA |
SRP217688 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE135502_RAW.tar |
1.6 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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