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Series GSE13645 Query DataSets for GSE13645
Status Public on Sep 22, 2009
Title Transcriptional profiling of p56lck-deficient regulatory and memory T cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Signaling through the T cell antigen receptor is essential for the formation of regulatory T (Treg) cells in the thymus and for their involvement in antigen-directed suppression of immune responses. Using a conditional null allele of the gene encoding p56Lck we show here that T cell antigen receptor (TCR) signaling is also essential for sustaining the phenotype and homeostasis of Treg cells. Inactivation of p56Lck in Treg cells resulted in large-scale changes in their gene expression profile, blocked their capacity to suppress responses, inhibited their proliferation, and caused them to redistribute in the body. The results make clear multiple aspects of the Treg cell phenotype that are dependent on a sustained capacity to respond through their TCRs.

Keywords: Genetic deficiency of p56lck
 
Overall design Two-condition experiment: wild-type memory or regulatory T cells versus lck-deficient memory or regulatory T cells.
 
Contributor(s) Killeen N, Klinger M
Citation(s) 19668367
Submission date Nov 18, 2008
Last update date Feb 17, 2015
Contact name Mark Klinger
E-mail(s) mark.klinger@ucsf.edu
Phone 415-502-5432
Organization name University of California, San Francisco
Department Microbiology and Immunology
Lab Nigel Killeen
Street address 513 Parnassus Avenue, Box0414
City San Francisco
State/province CA
ZIP/Postal code 94122
Country USA
 
Platforms (1)
GPL7656 Mouse Exonic Evidence Based Oligonucleotide (MEEBO) spotted microarrays
Samples (16)
GSM343477 R.lck2.vs.wt1
GSM343478 R.lck2.vs.wt1 dye swap
GSM343479 R.lck4.vs.wt3
Relations
BioProject PRJNA110195

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13645_RAW.tar 52.7 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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