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| Status |
Public on Nov 03, 2021 |
| Title |
Changes in gut microbiota induced by oral pathobiont deteriorate the pathology of non-alcoholic fatty liver disease |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Periodontitis increases the risk of non-alcoholic fatty liver disease (NAFLD). However, the precise mechanisms are unclear. Here, gut dysbiosis induced by orally administered Porphyromonas gingivalis, a representative periodontopathic bacterium, was implicated in the deterioration of NAFLD pathology. C57BL/6 mice were administered with the vehicle, P. gingivalis or Prevotella intermedia, with weaker periodontal pathogenicity, followed by feeding on a choline-deficient, high fat diet (CDAHFD60). CDAHFD60 feeding induced hepatic steatosis, and combined bacterial administration further aggravated NAFLD pathology with increased fibrosis. Liver gene expression analyses revealed that genes involved in the NAFLD pathology were perturbed with distinctive expression profiles induced by different bacteria. These differences may be due to quantitative and qualitative differences in the influx of gut bacterial products because the serum endotoxin level, gut microbiota composition, and serum metabolite profile caused by ingested P. intermedia and P. gingivalis were different. These findings provide insights into mechanisms linking periodontitis and NAFLD.
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| Overall design |
Effect of oral administration of P. intermedia and P. gingivalis on the liver gene expression profile was mesured in high fat diet-fed mice. Mice were divided into 4 groups ( regular chow, no bacteria (n=3), high fat diet, sham (n=4), high fat diet, P. intermdia (n=4), high fat diet, P. gingivalis (n=5)). Bacteria were administered 2 times aweek for 3 weeks nd high fat diet was fed for 2 weeks.
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| Contributor(s) |
Yamazaki K, Miura N, Okuda S, Yamazaki K, Kato T |
| Citation(s) |
34707622 |
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| Submission date |
Sep 05, 2019 |
| Last update date |
Nov 04, 2021 |
| Contact name |
Kazuhisa Yamazaki |
| E-mail(s) |
kaz@dent.niigata-u.ac.jp
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| Organization name |
Niigata University
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| Street address |
2-110-1 Gakkocho-dori, Chu-o-ku
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| City |
Niigata |
| ZIP/Postal code |
951-8514 |
| Country |
Japan |
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| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA564083 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE136937_RAW.tar |
61.4 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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