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Series GSE136994 Query DataSets for GSE136994
Status Public on Sep 30, 2021
Title Evaluation of the potential toxicity mechanism of single and combined exposure of dibutyl phthalate and diisobutyl phthalate to zebrafish (Danio rerio) testis based on transcriptomic analysis
Organism Danio rerio
Experiment type Expression profiling by high throughput sequencing
Summary Aquatic organisms are generally exposed to a mixture of phthalate esters (PAEs) that have been shown to induce reproductive toxicity. However, their potential toxicity mechanisms to aquatic organisms remain unclear. Here male zebrafish were exposed to dibutyl phthalate (DBP), diisobutyl phthalate (DiBP) and their mixtures for 30 days, and their effects on plasma sex hormones, testis histology and testis transcriptomics were investigated. DBP, DiBP and their mixtures could induce the disequilibrating ratio of testosterone (T) and plasma estradiol (E2) in plasma. The percentage of spermatozoa (Sz) was significantly decreased by 30.6% under DBP-1133 exposure and 27.8% under Mix-3 exposure, and widen intercellular spaces appeared under DiBP-1038 exposure. Transcriptome sequencing revealed 2795 differentially expressed genes (DEGs) in the DBP-1133 exposure group, 1613 DEGs in the DiBP-1038 exposure group and 4570 DEGs in the Mix-3 exposure group, indicating that the toxicity of combined exposure was higher than that of single exposure. Cytokine-cytokine receptor interaction was associated with the toxicity mechanism of DBP, DiBP and Mix. While GnRH signaling pathway and MAPK signaling pathway were related to the toxicity mechanism of DBP. ECM-receptor interaction, steroid hormone biosynthesis, retinol metabolism and PPAR signaling pathway were associated with the toxicity mechanism of DiBP and Mix.
Overall design Testis mRNA sequencing of adult female zebrafish after single and combined exposure to DBP and DiBP for 30 days
Contributor(s) Chen H, Feng W, Chen K, Qiu X, Xu H, Mao G, Zhao T, Ding Y, Wu X
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Submission date Sep 06, 2019
Last update date Oct 01, 2021
Contact name Hui Chen
Organization name jiangsu university
Street address Xuefu Road
City Zhenjiang
ZIP/Postal code 212013
Country China
Platforms (1)
GPL23085 HiSeq X Ten (Danio rerio)
Samples (4)
GSM4065042 CK2: control
GSM4065043 B2: dibutyl phthalate (DBP)
GSM4065044 I2: Diisobutyl phthalate (DiBP)
BioProject PRJNA564233
SRA SRP220636

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE136994_RAW.tar 1.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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