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| Status |
Public on Jun 21, 2020 |
| Title |
RNA-Seq and ChIP-Seq profiling of ELF3, an prion-like domain-containig in ELF3 that functions as a thermosensor in Arabidopsis. |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Temperature is a major environmental variable governing plant growth and development. ELF3 contains a polyglutamine (polyQ) repeat 8–10, embedded within a predicted prion domain (PrD). We find the length of the polyQ repeat correlates with thermal responsiveness. Plants from hotter climates appear to have lost the PrD domain, and these versions of ELF3 are stable at high temperature and lack thermal responsiveness. ELF3 temperature sensitivity is also modulated by the levels of ELF4, indicating that ELF4 can stabilise ELF3 function. This RNA-Seq dataset provides evidence for the hypothetical ELF3 function of temperature sensing .
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| Overall design |
Single samples were taken at each time point. RNA-Seqs and ChIP-Seqs were performed for different genotypes at different temperature and objective time.
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| Contributor(s) |
Jung J, Jaeger K, Geng F |
| Citation missing |
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| |
| Submission date |
Sep 11, 2019 |
| Last update date |
Jun 22, 2020 |
| Contact name |
Philip Wigge |
| E-mail(s) |
wigge@igzev.de
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| Organization name |
Cambridge University
|
| Department |
Sainsbury Laboratory
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| Street address |
47 Bateman street
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 1LR |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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| Samples (64)
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| Relations |
| BioProject |
PRJNA564976 |
| SRA |
SRP221358 |
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