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| Status |
Public on Aug 18, 2021 |
| Title |
Translation switch from initiation to elongation needs Not4 and Not5 collaboration |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Not4 and Not5 are crucial components of the Ccr4-Not complex with pivotal functions in mRNA metabolism. Both associate with ribosomes but mechanistic insights on their function remain elusive. Here we determine that Not5 and Not4 synchronously impact translation initiation and Not5 alone alters translation elongation. Deletion of Not5 causes elongation defects in a codon-dependent fashion, increasing and decreasing the ribosome dwelling occupancy at minor and major codons respectively. This enhanced difference in codon translation velocities alters translation globally and enables normally kinetically unfavorable processes such as nascent chain deubiquitination to take place. In turn this leads to abortive translation and favors protein aggregation. These findings highlight the global impact of Not4 and Not5 in controlling the programmed local speed of mRNA translation.
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| Overall design |
Assessment of translation through ribosome footprinting, SILAC and tRNA abundance and charging levels
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| Contributor(s) |
Allen G, Panasenko O, Collart M, Ignatova Z, Villanyi Z, Zagatti M, Polte C |
| Citation missing |
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| Submission date |
Sep 17, 2019 |
| Last update date |
Jun 27, 2022 |
| Contact name |
George Allen |
| Organization name |
University of Geneva
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| Street address |
Rue Michel-Servet 1
|
| City |
Geneve |
| State/province |
Geneve |
| ZIP/Postal code |
1206 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA566006 |
| SRA |
SRP222103 |
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