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Series GSE137978 Query DataSets for GSE137978
Status Public on Oct 22, 2019
Title GR cistromes reprogramming by High Fat Diet [ChIP-seq: GR DEX]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We mapped the genome-wide binding profiles of GR by using ChIP-Seq in livers from mice fed control or HFD diet after acute exogenous ligand (DEX) administration.
Overall design Male C57BL/6J mice were fed either control diet (11 kcal% fat w/sucrose, Research Diets D12329) or High Fat Diet (58 kcal% fat w/sucrose, Research Diets D12331) for 12 weeks. At 17 weeks of age, Dexamethasone (Sigma, D2915) was administered at ZT0 and at ZT12 as a single i.p. injection of 10mg/kg. Mice were sacrificed by cervical dislocation 1h later. ChIP was performed as previously described (Uhlenhaut et al. 2012). Sheared chromatin was immunoprecipitated with two different anti-GR antibody (Santa Cruz, sc-1004X and ProteinTech, 24050-AP). Two replicates per condition and time point were used, for a total of 12 GR (ZT0,ZT12, control/HFD) ChIP-seq samples.
Contributor(s) Mir AA, Quagliarini F
Citation(s) 31706703
Submission date Sep 25, 2019
Last update date Jan 21, 2020
Contact name Nina Henriette Uhlenhaut
Organization name HelmholtzZentrum München - IDC
Department Molecular Endocrinology
Lab Uhlenhaut IDC
Street address Ingolstädter Landstraße 1, Neuherberg
City Munich
State/province Bavaria
ZIP/Postal code D-85764
Country Germany
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (12)
GSM4096048 DL8pt: DEX_ZT12 control REP1
GSM4096049 DL9pt: DEX_ZT12 control REP2
GSM4096050 DH8pt: DEX_ZT12 HFD REP1
This SubSeries is part of SuperSeries:
GSE108690 Cistromic reprogramming of the diurnal glucocorticoid hormone response by high fat diet
BioProject PRJNA574072
SRA SRP223242

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE137978_RAW.tar 5.2 Mb (http)(custom) TAR (of NARROWPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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