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| Status |
Public on Oct 22, 2019 |
| Title |
GR cistromes reprogramming by High Fat Diet [ChIP-seq: GR DEX] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We mapped the genome-wide binding profiles of GR by using ChIP-Seq in livers from mice fed control or HFD diet after acute exogenous ligand (DEX) administration.
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| Overall design |
Male C57BL/6J mice were fed either control diet (11 kcal% fat w/sucrose, Research Diets D12329) or High Fat Diet (58 kcal% fat w/sucrose, Research Diets D12331) for 12 weeks. At 17 weeks of age, Dexamethasone (Sigma, D2915) was administered at ZT0 and at ZT12 as a single i.p. injection of 10mg/kg. Mice were sacrificed by cervical dislocation 1h later. ChIP was performed as previously described (Uhlenhaut et al. 2012). Sheared chromatin was immunoprecipitated with two different anti-GR antibody (Santa Cruz, sc-1004X and ProteinTech, 24050-AP). Two replicates per condition and time point were used, for a total of 12 GR (ZT0,ZT12, control/HFD) ChIP-seq samples.
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| Contributor(s) |
Mir AA, Quagliarini F |
| Citation(s) |
31706703 |
| |
| Submission date |
Sep 25, 2019 |
| Last update date |
Jan 21, 2020 |
| Contact name |
Nina Henriette Uhlenhaut |
| E-mail(s) |
henriette.uhlenhaut@helmholtz-muenchen.de
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| Organization name |
HelmholtzZentrum München - IDC
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| Department |
Molecular Endocrinology
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| Lab |
Uhlenhaut IDC
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| Street address |
Ingolstädter Landstraße 1, Neuherberg
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| City |
Munich |
| State/province |
Bavaria |
| ZIP/Postal code |
D-85764 |
| Country |
Germany |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE108690 |
Cistromic reprogramming of the diurnal glucocorticoid hormone response by high fat diet |
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| Relations |
| BioProject |
PRJNA574072 |
| SRA |
SRP223242 |
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