|
| Status |
Public on Oct 18, 2019 |
| Title |
PRRX1 overexpression in MDA-MB-231 breast cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To investigate downstream targets of PRRX1, we used MDA-MB-231 (MDA231) breast cancer cells which express low level of PRRX1 to generate a stable cell line where human PRRX1 was ectopically overexpressed
(MDA231-PRRX1), and performed comparative microarray analyses. Interestingly, we found many miRNAs that were upregulated in MDA231-PRRX1 cells.
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| |
|
| Overall design |
MDA231 cells were transduced with lentiviral vectors containing PRRX1, GFP and puromycine resistance cassette, and were selected with antibiotics. Pool of stably transduced cells were used for further analyses and microarray.
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| |
|
| Contributor(s) |
Ocaña OH |
| Citation(s) |
31712603 |
| |
| Submission date |
Sep 26, 2019 |
| Last update date |
Jan 17, 2020 |
| Contact name |
Angela Nieto |
| E-mail(s) |
anieto@umh.es
|
| Organization name |
Instituto de Neurociencias (CSIC-UMH)
|
| Department |
Developmental Neurobiology
|
| Lab |
Cell Movements in Development and Disease
|
| Street address |
Av. Ramón y Cajal s/n
|
| City |
San Juan de Alicante |
| State/province |
Alicante |
| ZIP/Postal code |
03550 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA574460 |
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